Figures & data
Table 1 Bacterial strains and plasmids used in this study
Figure 1 Salmonella effectors inhibit host cell exocytosis. A SEAP assay was performed on 293T cells transfected with plasmids encoding the indicated type III effectors. The protein secretion index is displayed. Values are the mean±SD for three independent experiments. Asterisks indicate a statistical significant difference compared to the empty vector.
Figure 2 The 4-phosphatase activity is necessary for SopB inhibition of exocytosis. SEAP assays were performed on 293T cells transfected with pEGFP-N2 plasmids encoding the indicated forms of SopB. sopB mutation abbreviations: C460S (C/S), R468A (R/A), and K530A (K/A). The protein secretion index is displayed. Values are the mean±SD for three independent experiments. Asterisks indicate a statistical significant difference compared to vector.
Figure 3 PI4K IIβ prevents SopB-mediated exocytosis inhibition. SEAP assays were performed on 293T cells transfected with pSEAP, either the pEGFP-N2 or pEGFP-N2-SopB plasmids, and one of the phosphoinositol kinase or phosphatases listed. The protein secretion index is displayed. Values are the mean±s.d. for three independent experiments. Asterisks indicate a statistical significant difference compared to cells transfected with vector only.
Figure 4 SopB delays transport of VSVG between the ER and Golgi. Immunoblot analysis of VSVG in cells cotransfected with ts045VSVG-GFP and either pEGFP-N2 (control), pEGFP-N2-SopB (SopB) or pEGFP-N2-SopBc/s (SopBc/s) at various time points after the shift to 32 °C. Endoglycosidase H (Endo H)-treated (+) and mock-treated (−) samples are indicated. The mobility of the Endo H-resistant (R; upper band) and -sensitive (S; lower band) forms of the protein is indicated. The boxes in B and C indicate the different Endo H sensitivity pattern exhibited by SopB-transfected cells. Actin immunoblotting is included as a loading control.
