Characterization of a G1P[8] rotavirus causing an outbreak of gastroenteritis in the Northern Territory, Australia, in the vaccine era

Figures & data

Table 1 Fluorescent focus reduction neutralization assay

Strain	RV4:1	RV4:2	RV4:3	RV4:5	F45:4	RV4 (poly)
RV4	720 000	125 000	210 000	125 000	12 000	78 000
D	41 000	3783	330 000	<100	ND	59 000
F45	ND	ND	ND	ND	1800	ND
V474	137 000	29 333	<100	327	30 000	230
ROTARIX	295 000	4867	203 667	2417	593	40 667

Abbreviation: ND, not done.

Neutralization titers are reported as the reciprocal dilution, which results in a 50% fluorescent focus reduction. Titers are representative of three independent assays.

Figure 1 A surface representation of the VP7 trimer (PDB ID: 3FMG). Each trimer is colored a different shade of gray and the antigenic regions A, C and F are colored light blue, dark blue and mid blue, respectively. The surface exposed amino acid residues that differ between the 2010 G1P[8] Alice Springs outbreak strain and Rotarix^® vaccine strain are shown in red.

Figure 2 Alignment of VP7 gene of the prototype G1P[8] strains RV4, D, the 2010 G1P[8] Alice Springs outbreak strain and Rotarix^® vaccine strain. Amino acid differences between these strains are shaded. Residues comprising the antigenic regions are defined within in brackets. The sites shown to escape neutralization with monoclonal antibodies include RV4:1 (sites 147 and 148, filled circle), RV4:2 (sites 213, filled square), RV4:3 (site 94, filled triangle) and RV4:5: (sites 147, 148 and other undefined sites, filled right pointing triangle).