Figures & data
Figure 1 Survival of zebrafish infected with graded doses of L. hongkongensis by immersion following dermal abrasion and intraperitoneal injection. (A) Survival curve of zebrafish after L. hongkongensis infection by immersion following dermal abrasion. (B) Plot of zebrafish mortality after L. hongkongensis infection by immersion following dermal abrasion against logarithm of infected doses of L. hongkongensis. (C) Survival curve of zebrafish after L. hongkongensis infection by intraperitoneal injection. (D) Plot of zebrafish mortality after L. hongkongensis infection by intraperitoneal injection against logarithm of infected doses of L. hongkongensis. All results are expressed as mean of three experiments.
Figure 2 Zebrafish after L. hongkongensis infection. (A and C) Lateral and ventral views of zebrafish two days after L. hongkongensis infection by immersion following dermal abrasion, showing abdominal hemorrhage and marked swelling with ascites. (B and D) Lateral and ventral views of zebrafish two days after E. coli infection by immersion following dermal abrasion.
Figure 3 Histopathological changes in liver sections of zebrafish two days after L. hongkongensis infection by immersion following dermal abrasion. (A) Necrosis of hepatocytes and infiltration of inflammatory cells around the central vein. (B) Structural disorder of hepatic lobules with detachment of several groups of hepatocytes; necrosis and apoptosis of hepatocytes and dilatation of hepatic sinusoid with abundant inflammatory cells. (C) Hydropic degeneration with swollen and edematous hepatocytes. (D) No obvious changes were observed in liver sections of zebrafish two days after E. coli infection by immersion following dermal abrasion.
Figure 4 Identification of bacterial colonies from ascitic fluid and homogenized tissues of zebrafish infected with L. hongkongensis by immersion following dermal abrasion using L. hongkongensis-specific primers. Lane M, DL1000 DNA marker; lane 1, DNA extracted from L. hongkongensis HLHK9; lanes 2 and 3, ascitic fluid of two zebrafish infected with E. coli by immersion following dermal abrasion; lane 4, liver of zebrafish infected with E. coli by immersion following dermal abrasion; lanes 5–7, ascitic fluid of three zebrafish infected with L. hongkongensis by immersion following dermal abrasion; lanes 8 and 9, liver of zebrafish infected with L. hongkongensis by immersion following dermal abrasion.
