Phylogenetic analysis of the major causative agents of hand, foot and mouth disease in Suzhou city, Jiangsu province, China, in 2012–2013

Figures & data

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Table 1 PCR and sequencing primers used in this study

Primer	Region		Sequence (5′–3′)	Position	Reference
PanEV-5′-UTR	Outer-primer
	Sense 59F	5′-UTR	5′-CYT TGT GCG CCT GTT TT-3′	59–75	^Citation21
	Antisense 588R	5′-UTR	5′-ATT GTC ACC ATA AGC AGC C-3′	588–570	^Citation21
	Inner-primer
	Sense 153F	5′-UTR	5′-CAA GYA CTT CTG TMW CCC C-3′	153–170	^Citation21
	Antisense 541R	5′-UTR	5′-CCC AAA GTA GTC GGT TCC-3′	541–524	^Citation21
PanEV-VP1	Outer-primer
	Sense 224	VP1	5′-GCI ATG YTI GGI ACI CAY RT-3′	1977–1996	^Citation22
	Antisense 222	VP1	5′-CIC CIG GIG GIA YRW ACA T-3′	2969–2951	^Citation22
	Inner-primer
	Sense AN89	VP1	5′-CCA GCA CTG ACA GCA GYN GAR AYN GG-3′	2602–2627	^Citation22
	Antisense AN88	VP1	5′-TAC TGG ACC ACC TGG NGG NAY RWA CAT-3′	2977–2951	^Citation22

Table 2 Demographics of all participants (n=109)

Group	n (%)
Age (years)
<1	7 (6.4%)
1–2	40 (36.7%)
2–3	27 (24.8%)
3–4	16 (14.7%)
4–5	14 (12.8%)
>5	5 (4.6%)
Gender
Male	72 (66.1%)
Female	37 (33.9%)

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Ben-Chetrit E, Wiener-Well Y, Shulman LM et al.Coxsackievirus A6-related hand foot and mouth disease: skin manifestations in a cluster of adult patients. J Clin Virol2014;59: 201–203.

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Ge S, Yan Q, He S, Zhuang S, Niu J, Xia N.Specific primer amplification of the VP1 region directed by 5′ UTR sequence analysis: enterovirus testing and identification in clinical samples from hand–foot–and–mouth disease patients. J Virol Methods2013;193: 463–469.

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