Figures & data
a, b Fold increases in viral RNA of EV71 subgenotype strains C1 (C1 91-480), C2 (2485), C4 (75-Yamagata), C5 (209-VN), B3 (SK-EV006), and B4 (C7-Osaka) in donors N39 and N41, determined by RT-PCR on lysed organoids. Data present the mean fold increase in two infection experiments + standard error of the mean (SEM). c Viral titers detected in the medium covering the gel-embedded organoids at 72 h postinfection (p.i.). Titers are expressed as the mean 50% cell culture infective dose (CCID50)/ml in two infection experiments + SEM
![Fig. 1 Replication kinetics of enterovirus 71 (EV71) in human airway organoids.a, b Fold increases in viral RNA of EV71 subgenotype strains C1 (C1 91-480), C2 (2485), C4 (75-Yamagata), C5 (209-VN), B3 (SK-EV006), and B4 (C7-Osaka) in donors N39 and N41, determined by RT-PCR on lysed organoids. Data present the mean fold increase in two infection experiments + standard error of the mean (SEM). c Viral titers detected in the medium covering the gel-embedded organoids at 72 h postinfection (p.i.). Titers are expressed as the mean 50% cell culture infective dose (CCID50)/ml in two infection experiments + SEM](/cms/asset/1eda5e50-1051-4dc7-bbe0-3fa39b2e52cc/temi_a_12039984_f0001_ob.jpg)
Residue 145 of the VP1 capsid region is marked by a box
![Fig. 2 Amino acid sequence comparison of the EV71 strain capsid regions included in the current study.Residue 145 of the VP1 capsid region is marked by a box](/cms/asset/a02ce39d-a32a-497d-bd99-98817c20c114/temi_a_12039984_f0002_ob.jpg)
a, b Fold increases in viral RNA of EV71 strains in donors N39 and N41, respectively. Values presented were calculated from RT-PCR assays of lysed organoids. c, d Viral titers detected in the medium covering embedded organoids of donors N39 and N41, respectively, at 0 to 72 h postinfection. Titers are expressed as the 50% cell culture infective dose (CCID50)/ml
![Fig. 3 Replication kinetics of EV71 subgenotype C1 strains with a glutamine (Q) or glutamic acid (E) at VP1 residue 145 in human airway organoids. a, b Fold increases in viral RNA of EV71 strains in donors N39 and N41, respectively. Values presented were calculated from RT-PCR assays of lysed organoids. c, d Viral titers detected in the medium covering embedded organoids of donors N39 and N41, respectively, at 0 to 72 h postinfection. Titers are expressed as the 50% cell culture infective dose (CCID50)/ml](/cms/asset/d1d2f235-d662-428f-bbd6-7271e6329767/temi_a_12039984_f0003_ob.jpg)
Data represent the mean 50% cell culture infective dose (CCID50)/ml in two infection experiments + SEM
![Fig. 4 Growth characteristics of EV71 C1, C2, and B3 strains with a glutamic acid (E), glycine (G), or glutamine (Q) at VP1-145, generated by site-directed mutagenesis, in rhabdomyosarcoma (RD) cells.Data represent the mean 50% cell culture infective dose (CCID50)/ml in two infection experiments + SEM](/cms/asset/243f06a7-7b2c-4d9a-b238-554d21873549/temi_a_12039984_f0004_ob.jpg)
a Fold increases in viral RNA of C1, C2, and B3 strains with VP1-145E, -G, or -Q in donor N41, determined by RT-PCR assay on lysed organoids. Data present the mean fold increase in two infection experiments + SEM. b Viral titers detected in the medium covering embedded organoids at 0–72 h postinfection. Titers are expressed as the mean 50% cell culture infective dose (CCID50)/ml in two infection experiments+SEM
![Fig. 5 Replication kinetics of EV71 strains with a glutamic acid (E), glycine (G), or glutamine (Q) at VP1-145, generated by site-directed mutagenesis, in human airway organoids.a Fold increases in viral RNA of C1, C2, and B3 strains with VP1-145E, -G, or -Q in donor N41, determined by RT-PCR assay on lysed organoids. Data present the mean fold increase in two infection experiments + SEM. b Viral titers detected in the medium covering embedded organoids at 0–72 h postinfection. Titers are expressed as the mean 50% cell culture infective dose (CCID50)/ml in two infection experiments+SEM](/cms/asset/e7b694c2-4b66-46cc-ab6b-3bd2fc4a643d/temi_a_12039984_f0005_ob.jpg)