A cross-sectional seroepidemiology study of EV-D68 in China

Figures & data

Fig. 1 Cytopathic effects observed in RD cells (×10).

a Control. b Cells infected with the synthetic virus. c Western blotting using anti-EV-D68 VP1 antibodies. Lane1: marker; lane2: cell control; lane3: Fermon virus; lane4: synthetic virus

Fig. 2 Comparison of the synthetic virus and the Fermon strain in NtAbs using 50 adult serum samples.

a Comparison of the NtAbs of anti-EV-D68 against the synthetic virus and Fermon (p = 0.0378). b The correlation of NtAbs against EV-D68 between the synthetic virus and Fermon (r = 0.444)

Seroprevalence rates and GMTs of NtAbs from the blood samples of 20 pairs of prenatal women and their neonates

Subjects	Seroprevalence rates	GMTs	95% CI
Prenatal women	100% (20/20)	168.0	93.6–301.7
Neonates	100% (20/20)	162.3	89.9–293.1

Relationship of EV-D68 NtAbs in blood samples collected from prenatal women and their neonates

The titer of prenatal women	The titer of neonates			Total titer
	1:8–1:64	1:96–1:512	>1:512
1:8–1:64	8(20%)	2(5%)	–	10(25%)
1:96–1:512	2(5%)	20(50%)	2(5%)	24(60%)
>1:512	–	2(5%)	4(10%)	6(15%)
Total	10(25%)	24(60%)	6(15%)	40(100%)

Fig. 3 Seroprevalence rate and GMTs of anti-EV-D68 in blood samples of 0-year-old to 15-year-old children.

a EV-D68 NtAb prevalence (100%) by age group; “n” was the number of the serum samples. b The GMT (positive serum) and seroprevalence rates were evaluated in different age groups

Seroprevalence rates and GMTs of EV-D68 neutralizing antibodies among age groups

	1mo–5mo	6mo–1yr	2yr–5yr	6yr–15yr
GMT	25.2(21.0–30.2)^a	15.0 (12.0–19.0)	24.7(17.15–35.6)^b	71.9(50.8–101.8)^c
Seroprevalence rates (%)	79(96/121)	20(33/109)	44 (34/77)	83(65/78)

^aThe seropositive rate of anti-EV-D68 in 6-month-old to 12-month-old infants was obviously lower than that in 1-month-old to 5-month-old infants, p = 0.0084

^bThe seropositive rate of anti-EV-D68 in 6-month-old to 12-month-old infants was obviously lower than that in 2-year-old to 5-year-old children, p = 0.0097

^cThe seropositive rate of anti-EV-D68 in 6-month-old to 12-month-old infants was obviously lower than that in 6-year-old to 15-year-old children, p = 0.0038