An emerging novel goose astrovirus associated with gosling gout disease, China

Figures & data

Fig. 1 Gross pathologic changes in diseased goslings.

a Deposit of urate on the surface of heart and liver. b Deposit of urate on the glandular gastric mucosa. c Deposit of urate on the trachea mucous membrane. d Deposit of urate in the leg joint. e Renal enlargement. f Deposit of urate in the gallbladder

Primers used in this study for detection of the viruses

Primer name	Sequence (5′–3′)	Target	Amplicon size	Reference
FAdV F	CAACTACATCGGGTTCAGGGATAACTTC	Hexon gene of fowl adenovirus	766bp	^Citation24
FAdV R	CCAGTTTCTGTGGTGGTTGAAGGGGTT
GHPV F	GAGGTTGTTGGAGTGACCACAATG	VP1 gene of goose hemorrhagic polyomavirus	144bp	^Citation25
GHPV R	ACAACCCTGCAATTCCAAGGGTTC
GRV F	TGAGACGCCTGACTACGATT	Sigma C gene of goose reovirus	380bp	^Citation26
GRV R	ATGCTTGGAGTGAGACGACT
GCoV F1	TATATCTGCAAAGAATAGGGCTCG	ORF1b gene of goose coronavirus	208bp	^Citation27
GCoV R1	GCCCATAAGCATAGGATCGTCAACG
GCoV F2	TGGCTCCAGGAAAAGATGTTGA	NC gene of goose coronavirus	495bp	^Citation27
GCoV R2	CTCCCCAATTCACTCTATCTG
DTMUV F	GCCACGGAATTAGCGGTTGT	E gene of duck Tembusu virus	401bp	^Citation28
DTMUV R	TAATCCTCCATCTCAGCGGTGTAG
GPVF	AGACTTATCAACAACCATCAT(C) T	VP1 gene of goose parvovirus	779bp	^Citation29
GPVR	TCACTTATTCCTGCTGTAG
AvAstV F	GAYTGGACNMGNTAYGAYGGNACNATNCC	ORF1b gene of avian astrovirus	434bp	^Citation30
AvAstV R	YTTNACCCACATNCCRAA
GSP1	gattacgccaagcttCATATCTATGACGACATTCGTGTC	Gene specific primer targeting to RdRp for 5′-RACE	/	This study
NGSP1	gattacgccaagcttCCTGTCCAACCATCATAAGACACC
GSP2	gattacgccaagcttAAGCCTCTCTTCTGGCGGATAC	Gene specific primer targeting to RdRp for 3′-RACE	/	This study
NGSP2	gattacgccaagcttGACACAAGCCTATCATCGCCATAG

Fig. 2 Phylogenetic tree analysis by MEGA7.

Amino acid sequence encoded by ORF2 of avastrovirus GD labeled with star

Fig. 3 Identification of the novel goose astrovirus GD isolate in LMH cells by indirect immunofluorescence assay.

a, b LMH cells infected with or without GD were reacted with the convalescent sera from the survival geese with gout symptomrespectively. c, d LMH cells infected with or without GD were reacted with the mouse sera against capsid P2 of GD respectively

Fig. 4 Infection study with the novel goose astrovirus GD.

a Bodyweight changes of the infected goslings. b Viral titer in the tissues from the infected goslings. c Histopathologic changes in infected goslings. a Normal kidney from the non-infected gosling, b Kidney from the infected gosling with renal tubular necrosis, abscission of renal tubular epithelial cells and protein cast in renal tubules

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