Differential transmission of Asian and African Zika virus lineages by Aedes aegypti from New Caledonia

Figures & data

Zika virus strains used in this study

ZIKV strain	Country	Year of collection	Host origin	Lineage/Clade	Passage history	GenBank Accession number	References
NC-2014-843	New Caledonia	February 2014	Human	Asian/Pacific	Three passages^(a)	SRR5309456	Dupont-Rouzeyrol et al.^Citation14
NC-2014-5132	New Caledonia	April 2014	Human	Asian/Pacific	Four passages^(a)	SRR5309451	Dupont-Rouzeyrol et al.^Citation14
NC-2015-2391	New Caledonia	July 2015	Human	Asian/Pacific	Four passages^(a)	SRR5309449	Dupont-Rouzeyrol et al.^Citation14
SA-2016-18246	French Guiana	January 2016	Human	Asian/American	Twelve passages^(c, a)	KU758873	Enfissi et al. GenBank direct submission
AF-1947-MR766	Uganda	April 1947	Monkey	African	Unknown^(b, c, a)	KY989511	Faye et al.^Citation12
AF-1991-HD78788	Senegal	February 1991	Human	African	Unknown^(b, c, a)	KF383039	Faye et al.^Citation12
AF-2002-ArD 165 522	Senegal	October 2002	Aedes vittatus	African	Eight passages^(b, c, a)	KF383029	Faye et al.^Citation12

Passage were conducted with Vero E6 cells^(a), AP-61^(b) cells, or C636 cells^(c). All viral stocks used for vector competence studies were ultimately produced using Vero E6 cells

Fig. 1 Infection, dissemination, and transmission of Aedes aegypti from New Caledonia with ZIKV belonging to the Asian lineage (Pacific and American clades).

Infection rate (a), dissemination rate (b) and transmission rate (c) at 6, 9, 14, and 21 days post-infection. The number of positive mosquitoes are indicated above each bar plot. Significant differences are indicated by asterisks (*p < 0.05; **p < 0.01; and ***p < 0.001). The bars indicate the 95% confidence interval for each ZIKV strain. NT indicates that females were not tested for this analysis point

Fig. 2 Infection, dissemination and transmission of Aedes aegypti from New Caledonia with ZIKV belonging to the African lineage

Infection rate (a), dissemination rate (b) and transmission rate (c) at 6, 9, 14, and 21 days post-infection. The number of positive mosquitoes are indicated above each bar plot. Significant differences are indicated by asterisks (*p < 0.05, **p < 0.01, and ***p < 0.001). The bars indicate the 95% confidence interval for each ZIKV strain. NT indicates that females were not tested for this analysis point

Fig. 3 Comparison of ZIKV strain transmission by Aedes aegypti from New Caledonia.

a Transmission efficiency of ZIKV by NC Ae. aegypti at 6, 9, 14, and 21 days post-infection. The number of positive mosquitoes are indicated above each bar plot. Significant differences are indicated by asterisks (*p < 0.05, **p < 0.01, and ***p < 0.001), in red for differences with the strain AF-1947-MR766 and in orange for the differences with the strain AF-1991-HD78788. b Saliva viral loads of each ZIKV strain at 6, 9, 14, and 21 days post-infection. The bars indicate the confidence interval of the mean for each ZIKV strain

Dupont-RouzeyrolMZika virus evolution on the edges of the Pacific oceanEmerg. Microbes Infect.2017610.1038/emi.2017.102

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FayeOMolecular evolution of Zika virus during its emergence in the 20(th) centuryPLoS Negl. Trop. Dis.20148e263610.1371/journal.pntd.0002636

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