Determination of 1‐Naphthol with Denatured DNA–Modified Pretreated Glassy Carbon Electrode

Abstract

A highly sensitive electrochemical biosensor for the detection of trace amount of 1‐naphthol was designed. Acid‐denatured DNA were immobilized onto the pretreated glassy carbon electrode (GCE(ox)) surface. Two well‐defined oxidation peaks were observed on the denatured DNA‐modified GCE(ox) at about +0.80 V and +1.10 V (vs. Ag/AgCl) in 0.10‐M acetate buffer (pH 5.0). The peak current of the guanine residue decreased with increasing concentration of 1‐naphthol. The optimum experimental conditions for the detection of 1‐naphthol were explored, and the calibration was linear for 1‐naphthol in the range of 1.0×10⁻⁸−1.1×10⁻⁶ M, with a correlation coefficient of 0.998. The limit of detection (LOD) was 5.0×10⁻⁹ M (S/N=3).

Keywords:

• 1‐naphthol
• biosensor
• DNA‐modified electrodes
• pretreatment of glassy carbon electrode

We thank Anhui Provincial Natural Science Foundation (Grant No. 03044906) and the Natural Science Foundation of Anhui Provincial Education Department (Grant No. 2006KJ116B) for financial support.