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Analytical Letters Volume 40, 2007 - Issue 13
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BIOANALYTICAL

The Effect of Temperature and Standings Can Cause Deviations in Prostate Specific Antigen (PSA) Assays

Chiu‐Lan Hsieh Research Institute of Biomedicinal Science and Technology, Hungkuang University, Taichung Hsien, Taiwan; Department of Food and Nutrition, Hungkuang University, Taiching Hsien, Taiwan
,
Ching‐Yuan Chang Division of Medical Laboratory, Kuang‐Tien Community Hospital, Shalu County, Taichung, Taiwan
,
Wang‐Sheng Ko Department of Internal Medicine, Kuang‐Tien Community Hospital, Shalu County, Taichung, Taiwan
,
Bor‐Shah Chen Department of Urology, Kuang‐Tien Community Hospital, Shalu County, Taichung, Taiwan
,
Kuan‐Chou Chen Department of Urology, Taipei Medical University Hospital, Taipei Medical University, Taipei, TaiwanCorrespondence[email protected]
&
Robert Y. Peng Research Institute of Biomedicinal Science and Technology, Hungkuang University, Taichung Hsien, Taiwan; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan
Pages 2485-2496 | Received 06 Apr 2007, Accepted 01 Jun 2007, Published online: 29 Oct 2007
 
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Abstract

Blood samples were assayed for PSA values immediately after sampled or by standing for an assigned period. Variation of the free‐ (f), total‐ (t) values, and the free to total (f/t) ratios were determined. Mathematical models were used to interpret the phenomena of deviation. Smaller values of PSA values changed randomly with time and temperature of standings, resulting in varying f/t ratios, while larger initial PSA values were relatively unaffected to any significant extent.

Model interpreted that the changes of PSA values might be caused by higher temperature and time of standings, and conformational participation was also possible.

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