Abstract
A rapid and sensitive chemiluminescence method using flow-injection has been developed for the determination of an analgesic agent drug, piroxicam. The method is based on the chemiluminescence reaction of piroxicam with an acidic potassium permanganate and Ru(bipy)3 2+. The chemiluminescence intensity is greatly enhanced when quinine sulfate is used as a sensitizer. After optimization of the different experimental parameters, a calibration graph was obtained over a concentration range of 3.0 × 0−8–3.0 × 0−5 mol L−1 with the detection limit of 1.0 × 0−8 mol L−1. The relative standard deviation is 1.5% (n = 11) for the determination of 8.0 × 10−7 mol L−1 piroxicam. The proposed method was successfully applied to commercial tablets, spiked serum, and urine samples.
The authors acknowledge the support from the National Natural Science Foundation of China, the Science Foundation of Huazhong University of Science & Technology (0101013008), and the Science Foundation of Huaibei Coal Industry Teachers' College.
Notes
UV/Vis: ultraviolet and visible spectrophotometry; CAM: Combining Amperometry and Multicommutation; LC: liquid chromatography; HPLC: high-performance liquid chromatography; LC-MS/MS: liquid chromatography–tandem mass spectrometry method; CL: Chemiluminescence.
a From Zhuzhou Yashen Pharmaceutical Co., LTD. Zhuzhou, China. (H41022631).
Xi′an No 4. Pharmaceutical Co., LTD. Xi′an, China. (H61021109).
Gannan Pharmaceutical Co., LTD. Gannan,China. (H36021370).
Note. R.S.D relative standard deviation.
a From a healthy volunteer man in local hospital, Huaibei, China.
b Mean ± sd, Average of six determinations.
Note. R.S.D, relative standard deviation.
a From three healthy volunteers men in local hospital, Huaibei, China.