Carbamide Peroxide Determination in Tooth Whitening Using a Reagentless HRP-Biosensor

Abstract

A rapid and effective method for carbamide peroxide (CP) quantification in pharmaceuticals is proposed. The reagentless biosensor was prepared by using lyophilized turnip extract as horseradish peroxidase (HRP) enzyme source. The biosensor presented the best performance. The measurements were carried out in 0.1 mol L⁻¹ Pipes buffer (pH 6.0). At −100 mV vs. Ag|AgCl, a linear response range from 3.0 to 25.0 mmol L⁻¹ was obtained. The quantification and detection limits were 1.2 and 0.4 mmol L⁻¹, respectively, and the response time was 0.5 s. The biosensor repeatability, storage, and lifetime were excellent, allowing a satisfactory CP quantification in real pharmaceutical samples, when compared with those obtained by the official method.

Keywords:

• Amperometric biosensor
• brassica napus L
• carbamide peroxide
• HRP
• lyophilization procedure

Financial support from FAPESP and CNPq (Proc. 470025/2006-9) is greatly acknowledged. I. G. V. and M. D. P. T. S. are indebted to FAPESP for the fellowships.

Notes

^a Standard deviation for three replicates.

^b Standard deviation for four replicates.