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Analytical Letters Volume 42, 2009 - Issue 10
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RESONANCE LIGHT SCATTERING

Resonance Light Scattering Method to Determine Binding Ratio and Functional Affinity Constant of Antigen/Antibody Immunoreaction

Huawen Zhao Department of Chemistry, Third Military Medical University, Chongqing, ChinaCorrespondence[email protected]
,
Chengzhi Huang Laboratory of Molecular Chemistry and Chemobiology, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
,
Liping Wu Department of Chemistry, Third Military Medical University, Chongqing, China
,
Sufen Shen Laboratory of Molecular Chemistry and Chemobiology, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
&
Zhuhong Qin Laboratory of Molecular Chemistry and Chemobiology, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
Pages 1495-1508 | Received 12 Jan 2009, Accepted 09 Apr 2009, Published online: 18 Jun 2009
 
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Abstract

Based on the immunoreaction equation and the quality function law, a method of determining the binding ratio and the affinity constant of antibody/antigen immunoreaction has been developed by measuring resonance light scattering (RLS) signals of the polystyrene latex particles. According to the method, the binding ratio and affinity constant of HIgG binding with goat anti-HIgG equal 0.71 and 47.3 mL/mg; that is, 1 mol HIgG can be bound with 1.4 mol goat anti-HigG. This new method is much simpler and easier to carry out when studying immunoreaction kinetics and determining the binding ratio and affinity constant.

The authors are grateful for the support from the Chongqing Natural Science Foundation (CSTC, 2007BB0049) and the National Natural Science Foundation Committee of China.

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