Simultaneous Determination of Ten Active Components in Chinese Medicine “Huang-Lian-Shang-Qing” Tablets by High-Performance Liquid Chromatography Coupled with Photodiode Array Detection

Abstract

A simple and sensitive high-performance liquid chromatography coupled with a photodiode array detection (HPLC-PAD) method was investigated for the simultaneous determination of ten components (rutin, berberine, palmatine, baicalin, wogonoside, baicalein, wogonin, emodin, chrysophanol, and physcion) in “Huang-Lian-Shang-Qing” (HLSQ) tablets. The method was optimized and the mobile phase composed of methanol (A)-3% phosphoric acid (B) was used to elute the targets in a gradient elution mode. All the calibration curves, precisions, and recoveries were good. Then, this method was successfully used to determine the ten compounds in 33 batches of HLSQ tablets for quality control of this medicinal product.

Keywords:

• HPLC-PAD
• Huang-Lian-Shang-Qing tablets
• Quality control

Acknowledgments

This study was financially supported by the scientists’ fund (No. 2008E11SF223) of the Science and Technology Foundation of Dalian, China.

Notes

^a RT = Retention times of the test compounds.

^b PA = Peak areas of the test compounds.

N.D.: not detected.