A Flow Injection Chemiluminescence Method for the Determination of Protein Using Copper(II)-Alizarin Red S Complex as an Efficient Chemiluminescent Probe

Abstract

A sensitive flow injection chemiluminescence (CL) method is proposed for the determination of bovine serum albumin (BSA) using Copper(II)-Alizarin Red S (ARS) complex as an efficient chemiluminescent probe. The detection is based on the binding of the copper(II)-ARS complex to proteins and the catalytic activity of copper(II)-ARS in the luminol-H₂O₂ CL system. Under the selected conditions, the CL intensity is linear with the concentration of BSA in the range of 5.0 × 10⁻¹¹ to 1.0 × 10⁻⁹ mol · L⁻¹. The detection limit was 2.0 × 10⁻¹¹ mol · L⁻¹. The method is successfully applied to the determination of protein in urine.

Keywords:

• Bovine serum albumin
• Chemiluminescence
• Copper(II)-Alizarin Red S complex
• Flow-injection analysis

Acknowledgments

This paper was submitted as part of a Special Issue on Flow Injection Analysis.

The authors acknowledge the support from the National Natural Science Foundation of China (No. 20505004), Outstanding Adult-young Scientific Research Encouraging Foundation of Shandong Province (No. 2008BS03015), and the Doctoral Found of QUST (No. 0022235).