Monitoring of Altered Free Fatty Acid Metabolic Patterns in Rat Plasma Following Hemorrhagic Stroke

Abstract

To uncover the physiological changes occurring after hemorrhagic stroke, we analyzed 24 free fatty acids in plasma as tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry from control and intracerebral hemorrhage rats. Hematoma volume decreased, and hemorrhage-induced behavioral abnormalities recovered overtime. Altered fatty acid metabolism at 7 days after hemorrhagic stroke spontaneously recovered to normal control levels at 14 days. The deformed star patterns of hemorrhagic stroke group were readily distinguished from the tetracosagonal shape of the control mean. Thus, the present method may be used for monitoring of biochemical and physiological events in hemorrhagic stroke.

Keywords:

• Free fatty acids (FFAs)
• Hemorrhagic stroke
• Metabolomic study
• Star pattern recognition

Acknowledgments

This work was supported by 2007 grant from “Department of Medical Sciences, The Graduate School Ajou University School of Medicine” to Y. H. Ahn; the National Research Foundation of Korea Grant funded by the Korean Government (KRF-2008-314-E00183) to M. J. Paik; and the Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0093826) to G. Lee.

Notes

Bold ion was selected for quantification.

^a Mean values as composition (%) by each FFA concentration (µmol/l) ratio to the total FFA concentration.

^b Kruskall-Wallis test between of control group and ICH group after 7 days.

^c Kruskall-Wallis test between of control group and ICH group after 14 days.

^d Kruskall-Wallis test between 7 days and 14 days after ICH.

M.-J. Paik and J. Y. Shin contributed equally to this work.