Abstract
Some G-quadruplex-hemin complexes can be used as a type of DNAzymes with peroxidase activity, it can efficiently catalyze H2O2-mediated oxidation reactions. Herein, a simple, colorimetric, and homogenous sensor design strategy based on G-quadruplex-hemin DNAzymes was developed. This strategy takes advantages of the competition between a DNA/DNA duplex and a DNA/target complex, and the formation of catalytically active G-quadruplexes in the presence of targets. With this method, no labeled oligonucleotides are needed, and no expensive instruments are required. This strategy may be developed as a general approach for the analysis of many metal ions and aptamer substrates. In this paper, the feasibility of this strategy in Hg2+ and adenosine triphosphate (ATP) detection was studied, and results showed that this “turn-on” process enabled the selective detection of aqueous Hg2+ and adenosine triphosphate (ATP) using a simple colorimetric technique.
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Acknowledgments
This work was supported by the Natural Science Foundation of Tianjin (No. 08JCZDJC21200), the National Basic Research Program of China (No. 2011CB707700), and the National Natural Science Foundation of China (No. 20975055).
Notes
a Obtained from commercial purified drinking water.
b Obtained from commercial spring drinking water.
c Obtained from Haihe River in Tianjin.