Abstract
A new automated method is reported for the determination of H2O2 in real samples. The method is based on the quenching effect of the analyte on the reaction between tris(2-carboxyethyl)phosphine (TCEP) and Ellman's reagent (DTNB). All necessary steps were accomplished under flow conditions using a hybrid sequential injection (SI)/flow injection (FI) setup. The sensitivity was enhanced by applying a stopped-flow step (120 s) in order to promote the reaction between H2O2 and TCEP. The proposed analytical protocol was validated for linearity (10–75 µmol L−1), limits of detection (c L = 1.0 µmol L−1), quantitation (c Q = 3.3 µmol L−1), precision (s r = 1.3–1.7%), accuracy, and selectivity. It was then applied successfully to the analysis of H2O2 in spiked rainwater and snow samples.
Notes
a Using aqueous solutions.
b Not detected.
a Using aqueous solutions.