Sequential Injection Analysis of Ciclopirox Olamine Using Lanthanide-Sensitized Luminescence Detection

Abstract

Ciclopirox olamine is an antifungal agent widely used in dermatological formulations for topical application. The methods described for its determination usually present time-consuming sample treatment or require major human participation. Here we present a rapid flow system for the determination of ciclopirox olamine, based on the use of sequential injection analysis with terbium-sensitized luminescence detection. The reaction between terbium and the analyte is carried out on-line, recording the analytical signal at 300/545 nm (λ_ex/λ_em). A detection limit of 15 µg L⁻¹ and a R.S.D. of 3% (n = 10) were obtained. It is worth mentioning that the method here presented is the only one that uses a flow system for the determination of ciclopirox olamine.

The flow system was applied to the analysis of different pharmaceutical preparations available in the Spanish Pharmacopoeia. A recovery study was performed, obtaining satisfactory results. The high solution throughput obtained, 50 samples per hour, makes the method suitable for its implementation in pharmaceutical laboratories for routine analysis.

Keywords:

• Automation
• Nail lacquer
• Shampoo
• SIA
• Spray
• TSL

Acknowledgments

This article is part of a Special Issue Automated on Flow Injection Techniques organized by Dr. Paraskevas Tzanavaras of Aristotelian University of Thessaloniki, Greece.

The authors are grateful to the “Consejería de Innovación, Ciencia y Empresa (Junta de Andalucía),” Project P07-FQM-02673, for financial support.

Notes

^a Analyte concentration, 200 µg L⁻¹.

L.D.R. = Linear dynamic range; D.L. = Detection limit; Q.L. = Quantitation limit; MEKC˭Micellar electrokinetic capillary chromatography.

^a Means of three determinations.

^b 10 mg g⁻¹.

^c 80 mg g⁻¹.

^d 15 mg g⁻¹.

^e 10 mg g⁻¹.