Flow Injection/Sequential Injection Chromatography: A Review of Recent Developments in Low Pressure with High Performance Chemical Separation

Abstract

This review focuses on the developments of the various parts of instrumentation and the operation of low to medium pressure flow injection and sequential injection chromatography (FIC and SIC) systems. The report and discussion include solution delivery system, separation column, flow cell and detector, mobile phase management, and online sample pretreatment. Applications of FIC and SIC and their differences as compared to HPLC are also presented.

Acknowledgments

S. K. Hartwell and A. Kehling acknowledge The College of Arts and Sciences, Xavier University, for the financial support through summer research fund 2012. S. Lapanantnoppakhun and K. Grudpan also thank Chiang Mai University through the Center of Excellence for Innovation in Analytical Science and Technology.

Notes

DW = detection wavelength;IS = internal standard;V = sample volume; AT = analysis time; LR = linear range; DL = detection limit;LOQ = limit of quantitation;R = resolution;PBS = phosphate buffer solution; ACN = acetonitrile; MeOH = methanol; KHP˭Potassium hydrogen phthalate; THF = tetrahydrofuran; SPE = solid phase extraction.

This article is part of a Special Issue on Automated Flow Injection Techniques organized by Dr. Paraskevas Tzanavaras of Aristotelian University of Thessaloniki, Greece.