Facile Preparation of an Ionic Liquid Composite Mesoporous Polymer as a Solid Phase Extraction Adsorbent for the Separation and Purification of Flavonoids from Chamaecyparis obtusa

Abstract

A new ionic liquid composite mesoporous polymer (ILCMP) was synthesized by one-pot copolymerization. The obtained ILCMP with good morphology, good pore diameter distribution, and high adsorption capacity was successfully applied as the sorbent in solid-phase extraction (SPE) to extract and separate quercitrin and myricetin from Chamaecyparis obtusa. 87.8–101.8% of satisfactory recoveries were obtained with RSDs less than 6.2% and the amounts of quercitrin and myricetin in Chamaecyparis obtusa were 0.38 and 0.12 mg/g, respectively. The proposed ILCMP-SPE-HPLC method was applied for the separation, purification, and determination of flavonoids from natural products.

Keywords:

• Liquid chromatography
• Chamaecyparis obtusa
• Flavonoid
• Ionic liquid composite mesoporous polymer
• Solid-phase extraction

Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2012-0005250).