Abstract
Infrared spectroscopy with attenuated total reflectance was investigated for the rapid determination of atractylenolide I and atractylenolide III contained in crude and processed Atractylodes macrocephala. High performance liquid chromatography was used as a reference method for the analysis, and a multivariate calibration model based on a partial least squares algorithm was developed to correlate the spectra and values determined by the reference method. The results suggested that the relative deviation of the predicted contents of atractylenolide I and atractylenolide III in the samples were less than 5.0%, which proved the stability and reliability of the models used. The proposed method is fast and nondestructive, and provides a novel, efficient, and environmentally-friendly approach for the rapid determination of the active components in crude and processed traditional Chinese herbal medicines.
Notes
MSC = multiplicative scatter correction; SNV = standard normal variant.
MSC = multiplicative scatter correction; SNV = standard normal variant.
Gang Cao and Hao Cai contributed equally to this work.
The authors explicitly declare that this article has no conflict of interest.
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