Determination of Bisphenol A by a Gold Nanoflower Enhanced Enzyme-Linked Immunosorbent Assay

ABSTRACT

The use of nanomaterials to improve the sensitivity of enzyme-linked immunosorbent assays (ELISA) for small molecules allows wider application of this method. In this work, gold nanoflowers were synthesized by a seed-mediated procedure and used to prepare a horseradish peroxidase-immunoglobulin G-gold nanoflower complex. The complex was used as the signal amplification probe in a novel ELISA for bisphenol A. The gold nanoflower-enhanced ELISA demonstrated excellent detection sensitivity of 0.05 ng/mL, which was 20 times more sensitive than a standard ELISA procedure. A linear dynamic range from 0.1 to 5.0 ng/mL was obtained by this approach. The gold nanoflower-enhanced ELISA was used to determine bisphenol A in lake water, with recoveries from 92.0 to 102.0% in samples fortified at concentrations of 0.5, 1.0, and 3.0 ng/mL. The gold nanoflower-enhanced ELISA was shown to offer good sensitivity, simplicity, and low cost.

KEYWORDS:

• Bisphenol A
• enzyme-linked immunosorbent assays (ELISA)
• enzyme-linked immunosorbent assay
• gold nanoflowers

Acknowledgments

We acknowledge Professor Chuanlai Xu (Jiangnan University) for providing the anti-bisphenol A antibody.