Abstract
Au2Ni1 alloy nanoparticles (NPs) were synthesized by a reverse microemulsion procedure and the structure, morphology, and composition were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), and energy-dispersive X-ray spectroscopy (EDS). The results showed that the synthesized NPs were random alloys with a Au/Ni atom ratio of 2:1 with a face-centered cubic (fcc) structure and average diameters of 8 ± 2 nm. A novel hydrogen peroxide (H2O2) sensor was constructed using horseradish peroxidase (HRP) modified by the Au2Ni1 alloy NPs. The sensor properties were characterized by cyclic voltammetry (CV) and amperometry in H2O2 solution. The results demonstrate that the sensor exhibited good performance for H2O2 in phosphate buffer saline at pH 7.2. The detection limit was 2.12 × 10−8 mol/L with a linear correlation coefficient (R2) of 0.99647 with good stability and reproducibility.