Separation of anilines by a covalent triazine-triphenyl polymer as a stationary phase for their normal-phase and reverse-phase determination by high-performance liquid chromatography (HPLC)

Abstract

A covalent triazine-triphenyl polymer (CTP_CC-TP) was examined as a novel column packing material for high-performance liquid chromatography (HPLC). The separation performance of CTP_CC-TP was characterized using anilines as the analytes in reverse-phase (RP) and normal-phase (NP) modes. Various retention mechanisms, such as hydrogen-bonding, π-π, and hydrophobic-hydrophobic interactions, endowed CTP_CC-TP with a hydrophilic-hydrophobic balance performance, demonstrating promise for applications as a stationary phase for HPLC in normal-phase and reversed-phase. The CTP_CC-TP column showed good reproducibility with relative standard deviations from 0.28 to 0.93%, 1.73 to 3.12%, 1.57 to 3.69%, and 1.36 to 2.54% for retention time, peak height, peak area, and peak width at half maximum, respectively. In the normal and reverse phase modes, a thermodynamically spontaneous transfer process is demonstrated through negative ΔG values controlled by negative ΔH and ΔS values during the chromatographic separation. Excellent chemical and solvent stabilities, and the hydrophilic-hydrophobic balance performance make CTP_CC-TP a good candidate for use in HPLC.

Keywords:

• Anilines
• covalent triazine-triphenyl polymer
• high-performance liquid chromatography (HPLC)
• normal-phase
• reverse-phase

Additional information

Funding

The authors thank the Center of Advanced Analysis and Gene Sequencing and Genetics of Zhengzhou University for providing the experimental platform. This work is financially sponsored by grants from the National Natural Science Foundation of China (21974124, 22004109, 22036003).