Determination of Glucose by the Catalysis of Luminol Chemiluminescence Using One-Step Synthesized Platinum/Silver Nanoparticles as a Peroxidase Mimetic

Abstract

Bimetallic Pt/Ag nanoparticles (Pt/Ag NPs) were synthesized using 3-hydroxyphenyl-boronic acid as the reducing and stabilizing agent by a simple, one-step hydrothermal method. The bimetallic Pt/Ag NPs were demonstrated to possess intrinsic peroxidase mimetic catalytic activity for the luminol/H₂O₂ reaction to generate chemiluminescence (CL). The catalytic properties of the bimetallic Pt/Ag NPs were thoroughly investigated. The results showed that the combination of Pt and Ag greatly enhanced the chemiluminescence which is attributed to their synergy. Moreover, the prepared bimetallic Pt/Ag NPs exhibited superior peroxidase mimetic activity to horseradish peroxidase and were stable under a wide range of pH values and temperatures. Using the bimetallic Pt/Ag NPs as the catalyst, a novel chemiluminescence sensor was developed for the determination of glucose with a linear range from 1 to 500 µM and a detection limit of 0.35 µM. The developed method was successfully employed to determine glucose in human serum with satisfactory results. Bimetallic Pt/Ag NPs with peroxidase mimetic catalytic activity have promising applications for determination of glucose in clinical samples.

Keywords:

• Chemiluminescence
• glucose
• peroxidase mimetic
• platinum/silver nanoparticles (Pt/Ag NPs)

Disclosure statement

There are no conflicts of interest to declare.

Statement of ethical approval

Human serum samples collected from three volunteers were kindly provided by Changchun Xingtai Hospital (Changchun, China). This work was performed in accordance with a protocol approved by the local ethical committee. Informed consent was obtained from all donors prior to sample collection.

Additional information

Funding

This work was funded by the Doctoral Research Initiation Fund Project of Jilin Engineering Normal University (BSKJ202103) and Jilin Province Science and Technology Development Plan Project (20200403152SF).