Biofunctionalized Silicon Carbide-Titanium (SiC-Ti) Platforms for the Determination of Enteropathogenic Escherichia coli by an Infrared Biosensor

Abstract

Escherichia coli (E. coli) may be harmful to humans. Hence, a rapid, low cost, and easy detection method is needed, and optical biosensors are an excellent option. Here a new platform based upon silicon carbide and titanium (SiC-Ti) was used to determine enteropathogenic E. coli by Fourier transform infrared (FTIR) spectroscopy. Self-assembly monolayer (SAM) approach was used to modify the platform. Antibody anti-E. coli flagella were obtained and utilized as the biological recognition element. Three concentrations of bacteria (10, 100, and 1000 CFU/mL) were determined. The detection region was from 1330 to 1480 cm⁻¹, where characteristic bands associated with E. coli were determined. An increase in the absorption intensity was observed with the bacteria concentration, demonstrating suitability for quantitative analysis.

Keywords:

• Biosensor
• Escherichia coli
• Fourier transform infrared (FTIR) spectroscopy
• SiC-Ti

Disclosure statement

No conflicts of interest are reported by the authors.

Additional information

Funding

The authors thank Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT) for the postdoctoral scholarship (Project number 2709087) granted to the first author and for the scholarships given to Vázquez-Gutiérrez and Retana-González to study a doctorate in Biotechnology Science and a master’s degree in electronics program, respectively.