A genetic screen of the island races of Wren Troglodytes troglodytes in the North-east Atlantic

Figures & data

Table 1. List of samples used for phylogenetic analysis.

Accession Number	Taxon	Location	Date of collection	Identification number
HG423581	T. t. fridariensis	Fair Isle	24 April 2011	R: DLE511
HG423582	T. t. fridariensis	Fair Isle	08 October 1997	Found dead
HG423583	T. t. fridariensis	Fair Isle	15 October 1997	Found dead
HG423584	T. t. hebridensis	Hyskeir	07 April 1923	M: 1968.80.22
HG423585	T. t. hebridensis	Hyskeir	29 April 1924	M: 1968.80.20
HG423586	T. t. hebridensis	South Uist	25 June 1947	M: 1956.65.4764
HG423587	T. t. islandicus	N. Iceland	11 December 1911	M: 1934.61.1073
HG423588	T. t. islandicus	N. Iceland	13 April 1912	M: 1934.61.1079
HG423589	T. t. borealis	Faroe Islands	03 April 1949	M: 1953.53.16
HG423590	T. t. borealis	Faroe Islands	23 May 1972	M: 1898.4.17
HG423591	T. t. hirtensis	St Kilda	18 August 1999	M: 2000.10.2
HG423592	T. t. hirtensis	St Kilda	28 September 1911	M: No number
HG423593	T. t. zetlandicus	Virkie, Shetland	11 May 2011	R: BPE116
HG423594	T. t. zetlandicus	Virkie, Shetland	11 May 2011	R: BPE117
HG423595	T. t. zetlandicus	Unst, Shetland	06 November 2011	Found dead

Note: Identification numbers refer to museum accession numbers (M) or bird ring numbers (R).

Figure 1. The ranges of subspecies of Eurasian Wren sampled for this study.

Table 2. Primers used to sequence the ND2 gene in T. troglodytes subspecies.

Primer	Sequence 5′- 3′	5′ start site
L5216	GGCCCATACCCCGRAAATG	5198
H6313	ACTCTTRTTTAAGGCTTTGAAGGC	6313
TROGF1	TGAACCCCCAAGCAAAACTAG	5242 (2)
TROGR1	TGGAGAATAGGACTAGGGTG	5461 (221)
TROGF2	ACCCTAGTCCTATTCTCCAG	5442 (202)
TROGR2	GTAGGGTGGGGTTTAGTGAG	5701 (461)
TROGF3	CTCACTAAACCCCACCCTAC	5681 (441)
TROGR3	GYGGTTATAAGGGYGTAYAG	5879 (639)
TROGF4B	CCCAAACTTRCCCTRCTAAAC	5832 (592)
TROGR4	GCTGCTGGGGCTATTTCTTG	6077 (837)
TROGF5A	TCCCAAAATGACTCATCAATCC	6022 (782)
TROGR7	AATGGGGAGGATTAGGGGTG	6280 (1040)

Note: 5′ start sites refer to the position in the mitochondrion of Gallus gallus (Genbank accession number NC_001323); while 5′ start sites in parentheses refer to the position within the ND2 gene.

Table 3. Polymorphic nucleotide sites defining the 12 ND2 haplotypes resolved from the 33 T. troglodytes sequences examined. Sequences are given in relation to haplotype 1, where a dot indicates identity. The subspecies that each haplotype was found in is listed.

			1	1	1	2	2	3	5	5	6	6	7	7	8	9
	1	7	7	8	9	1	4	3	2	9	2	8	4	6	1	6
	2	5	4	1	5	0	9	3	5	0	1	5	4	8	6	5
1	A	C	A	T	T	C	A	C	C	G	A	A	C	C	G	A	T(11), Z(3), GB(3), B, F
2	.	.	G	C	C	.	.	.	.	.	.	.	.	.	.	.	K(2)
3	.	.	.	.	.	.	.	.	.	.	G	.	.	.	.	.	H(2)
4	.	.	G	.	.	.	.	T	.	.	.	.	.	.	.	.	I(2)
5	.	.	.	.	.	.	.	.	.	A	.	.	.	.	.	G	GB
6	.	.	.	.	.	.	.	.	.	.	.	.	.	.	A	.	GB
7	.	.	.	.	.	.	G	.	.	.	.	.	.	.	.	.	T
8	T	G	.	.	.	.	.	.	.	.	.	.	.	.	.	.	B
9	.	.	.	.	.	.	.	.	.	.	G	.	T	.	.	.	F
10	.	.	.	.	.	.	.	.	A	.	G	.	.	.	.	.	F
11	.	.	.	.	.	A	.	.	.	.	G	.	.	T	.	.	H
12	.	.	.	.	.	.	.	.	.	.	.	G	.	.	.	.	GB

Note: Numbers in parentheses denote the number of individuals from a subspecies that share the same haplotype, in cases where there are more than one. T = nominate troglodytes, GB = birds from mainland Great Britain (troglodytes/indigenus), Z = zetlandicus, B = borealis, F = fridariensis, K = hirtensis, H = hebridensis and I = islandicus.

Figure 2. Minimum evolution (a) and neighbour joining (b) phylogenies of 13 Troglodytes ND2 haplotypes based on the Tamura–Nei distance with a gamma shape parameter of 1.31. Maximum parsimony (c) phylogeny shows most parsimonious tree with length = 58. Numbers at nodes denote bootstrap confidence indexes > 30%. Haplotype numbers (as per Table 3) are given to the right of branch termini. T = nominate troglodytes, GB = birds from mainland Great Britain (troglodytes/indigenus), Z = zetlandicus, B = borealis and F = fridariensis.

Figure 3. Frequency distributions of nucleotide divergences between individuals of the same subspecies (white bars) and different subspecies (black bars).