Figures & data
Figure 1. Derivation and culture of chicken stem cells from various developmental stages. Chicken ESCs derived from the blastodermal cells at embryonic stage X. In the culture, cESCs grow on the STO feeder layer and attach to each other with visible borders. PGCs have the ability to be reprogrammed into EGC colonies (from 5.5–6 d old embryo) on the CEF feeder layer. Chicken iPSCs have been generated by in vitro reprogramming of chicken fibroblast cells (from 8–12 d old embryo) on MEF feeder layer.
ESCs: embryonic stem cells; STO: mouse fibroblast cell line; CEF: chicken embryonic fibroblast cells; MEF: mouse embryonic fibroblast cells; EGCs: embryonic germ cells; iPSCs: induced pluripotent stem cells.
Figure 2. Optimisation of culture conditions for suspension culture of chicken stem cells. Strategies for generating large-scale cultures using feeder-free and serum-free suspension culture.
