Proteomics analysis of preadipocytes between fat and lean broilers

ABSTRACT

1. Reducing excessive chicken body fat deposition is a main goal of the poultry industry. Preadipocytes are important in adipose tissue growth and development.

2. To discover proteins related to chicken fat deposition, two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) was used to identify differentially expressed proteins in preadipocytes derived from Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF).

3. A total of 46 differentially expressed protein spots were found in the preadipocytes between fat and lean broilers. Matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) analysis showed the protein spots corresponded to 33 different proteins. The proteins were mainly related to biological oxidation, cell proliferation, cytoskeleton, lipid metabolism, molecular chaperone, protein synthesis and signal transduction.

4. From the perspective of protein expression, these results lay a foundation for further study of the genetic mechanism of broiler adipose tissue growth and development.

KEYWORDS:

• 2-D DIGE
• broiler
• differentially expressed protein
• fat deposition
• preadipocyte
• proteome

Acknowledgments

The authors gratefully acknowledge the members of the Poultry Breeding Group at Northeast Agricultural University for the help in managing the birds and collecting the data. The authors thank International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [31201796]; China Agriculture Research System [CARS-42]; National 973 Project of China [2009CB941604]; National 863 Project of China [2013AA102501].