Direct detection of Salmonella from poultry samples by DNA isothermal amplification

ABSTRACT

1. Salmonellosis is one of the most important diseases in public health and it is usually associated with poultry product consumption. This study aimed to validate rapid methods to detect Salmonella spp. from poultry samples.

2. A DNA isothermal amplification method, previously developed for other matrices, was applied for the specific detection of Salmonella spp. from various samples, including poultry tissues, drag and boot swabs, faeces and feed. A new procedure was validated with Salmonella spp. serotypes and isolates from other enteric bacterial species, as well as naturally contaminated poultry samples.

3. The study demonstrated the successful development and implementation of a procedure, including a DNA isothermal amplification method, for the detection of Salmonella spp. directly from tissues, drag and boot swabs, faeces and feed. The whole procedure can be performed in less than 24 hours and it has been successfully used in a veterinary diagnostic laboratory.

KEYWORDS:

• Foodborne diseases
• bacteriological analysis
• outbreaks
• chicken
• salmonellosis

Acknowledgments

The authors thank the staff of Simbios Biotecnologia and Molecular Diagnosis Laboratory of ULBRA for technical support and the laboratories Porto Belo and Simbios Biotecnologia for providing the samples.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed here.

Additional information

Funding

This study was financed by Simbios Biotecnologia, FAPERGS (Fundação de Amparo à Pesquisa do Rio Grande do Sul) and FINEP (Financiadora de Estudos e Projetos). VRL was also financially supported by the National Council for Scientific and Technological Development from Brazil (CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico; process number 311010/2017-2). JW and MNS were supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.