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Caryologia
International Journal of Cytology, Cytosystematics and Cytogenetics
Volume 65, 2012 - Issue 3
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Article

Cytogenetic analysis of Miscanthus × giganteus and its parent forms

Andrzej Chramiec-Głąbik Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Cracow 31-044, Poland
,
Aleksandra Grabowska-Joachimiak Cytogenetics Group in the Department of Plant Breeding and Seed Science, University of Agriculture in Cracow, Cracow 31-140, Poland
,
Elwira Sliwinska Laboratory of Molecular Biology and Cytometry, Department of Genetics and Plant Breeding, University of Technology and Life Sciences, Bydgoszcz 85-789, Poland
,
Justyna Legutko Cytogenetics Group in the Department of Plant Breeding and Seed Science, University of Agriculture in Cracow, Cracow 31-140, Poland
&
Adam Kula Cytogenetics Group in the Department of Plant Breeding and Seed Science, University of Agriculture in Cracow, Cracow 31-140, PolandCorrespondence[email protected]
Pages 234-242 | Published online: 21 Nov 2012

Figures & data

Table 1. Sources of the research material.

Figure 1 Metaphase chromosomes in three Miscanthus genotypes, staining with acetic orceine. (A) M. sinensis M07 (2n  =   2x  =   38). (B) M. sacchariflorus M115 (2n  =   2x  =   38). (C) M.   ×  giganteus (2n  =   3x  =   57   +   3B). (D) M  ×  giganteus 2n  =   46. B-chromosomes arrowed. Scale bar   =   5 μm.

Figure 1 Metaphase chromosomes in three Miscanthus genotypes, staining with acetic orceine. (A) M. sinensis M07 (2n  =   2x  =   38). (B) M. sacchariflorus M115 (2n  =   2x  =   38). (C) M.   ×  giganteus (2n  =   3x  =   57   +   3B). (D) M  ×  giganteus 2n  =   46. B-chromosomes arrowed. Scale bar   =   5 μm.

Table 2. Karyotype structure in four studied Miscanthus genotypes.

Figure 2 Metaphase chromosomes in four Miscanthus genotypes, C-banding/DAPI staining. (A) M. sinensis M07 (2n  =   38). (B) M. sinensis cv. Gracillimus (2n  =   38). (C) M. sacchariflorus M115 (2n  =   38). (D) M.   ×  giganteus (2n  =   57). Satellite chromosomes (SAT) arrowed. Scale bar   =   5 μm.

Figure 2 Metaphase chromosomes in four Miscanthus genotypes, C-banding/DAPI staining. (A) M. sinensis M07 (2n  =   38). (B) M. sinensis cv. Gracillimus (2n  =   38). (C) M. sacchariflorus M115 (2n  =   38). (D) M.   ×  giganteus (2n  =   57). Satellite chromosomes (SAT) arrowed. Scale bar   =   5 μm.

Figure 4 Karyograms of two Miscanthus sinensis genotypes (2n  =   38), C-banding/DAPI staining. (A) M. sinensis M07. (B) M. sinensis cv. Gracillimus. Satellite chromosomes (SAT) indicated. Scale bar   =   5 μm.

Figure 4 Karyograms of two Miscanthus sinensis genotypes (2n  =   38), C-banding/DAPI staining. (A) M. sinensis M07. (B) M. sinensis cv. Gracillimus. Satellite chromosomes (SAT) indicated. Scale bar   =   5 μm.

Figure 5 Karyogram of Miscanthus sacchariflorus M115 genotype (2n  =   38), C-banding/DAPI staining. Satellite chromosomes (SAT) indicated. Scale bar   =   5 μm.

Figure 5 Karyogram of Miscanthus sacchariflorus M115 genotype (2n  =   38), C-banding/DAPI staining. Satellite chromosomes (SAT) indicated. Scale bar   =   5 μm.

Figure 3 Karyogram of Miscanthus  ×  giganteus (2n  =   57), C-banding/DAPI staining. Satellite chromosomes (SAT) indicated. Scale bar   =   5 μm.

Figure 3 Karyogram of Miscanthus  ×  giganteus (2n  =   57), C-banding/DAPI staining. Satellite chromosomes (SAT) indicated. Scale bar   =   5 μm.

Figure 6 Heterochromatin contribution to the karyotypes of four Miscanthus genotypes.

Figure 6 Heterochromatin contribution to the karyotypes of four Miscanthus genotypes.

Figure 7 DNA histograms of nuclear preparation from leaves of four Miscanthus genotypes. (A) M. sinensis M07 (2n  =   38). (B) M. sinensis cv. Gracillimus (2n  =   38). (C) M. sacchariflorus M115 (2n  =   38). (D) M.   ×  giganteus (2n  =   57). 1: peak G0/G1 of Miscanthus; 2: peak G0/G1 of internal standard (P. sativum, 9.11 pg/2C).

Figure 7 DNA histograms of nuclear preparation from leaves of four Miscanthus genotypes. (A) M. sinensis M07 (2n  =   38). (B) M. sinensis cv. Gracillimus (2n  =   38). (C) M. sacchariflorus M115 (2n  =   38). (D) M.   ×  giganteus (2n  =   57). 1: peak G0/G1 of Miscanthus; 2: peak G0/G1 of internal standard (P. sativum, 9.11 pg/2C).

Table 3. Statistical analysis of the nuclear DNA amount of four Miscanthus genotypes.

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