Karyological and morphological investigations on a Hieracium putatively endemic to the National Park “Foreste Casentinesi, M. Falterona, Campigna” (northern Apennines, central Italy)

Figures & data

Figure 1 Localization in Italy of the study area.

Figure 2 The plant in the wild (a whole plant with flowers; inset: a fruiting capitulum).

Figure 3 The original specimen on which Hieracium villosum var. zangherii was invalidly described.

Figure 4 A recent specimen of Hieracium dentatum subsp. xanthostylophorum (= “H. villosum var. zangherii”).

Figure 5 (a) Photomicrograph of mitotic metaphase plate of Hieracium dentatum subsp. xanthostylophorum (= “H. villosum var. zangherii”); (b) karyogram.

Table 1. Karyological parameters of some taxa belonging to Hieracium subgen. Hieracium.

Taxon	Geographical location	Label inscatter plots	TF%	A	A1	A2	Ask%	CVCI	CVCL	MCA	Average total length (μm)	Total length (μm)	Ploidy	2n	Karyotype formula(Levan et al. 1964)
H. dentatum subsp . xanthostylophorum	Italy, northern Apennines between Tuscany and Emilia	DX	37.31	0.25	0.38	0.16	62.7	18.41	16.46	25.4	4.35	156.75	4x	36	2M + 14m + 18sm + 2st
H. racemosum [1]	Italy, southern Tuscany	RA	36.4		0.41	0.16					6.21	108.3	2x	18	8m + 10sm
H. sabaudum [1]	Italy, southern Tuscany	SA	37.1		0.4	0.18					4.80	131.2	3x	27	9m + 18sm
H. umbellatum [1]	Italy, southern Tuscany	UA	35.5		0.44	0.17					5.6	150.6	3x	27	9m + 18sm
H. murorum [1]	Italy, southern Tuscany	MA	38		0.37	0.25					4.6	123.5	3x	27	3M + 9m + 15sm
H. grovesianum [1]	Italy, southern Tuscany	GA	37.8		0.38	0.22					5.05	131.3	3x	27	12m + 15sm
H. sabaudum [2] ^∗	Slovakia, western Carpathians	SC	40.33	0.19	0.30	0.16	59.60	23.05	15.81	19.3	3.76	101.50	3x	27	6M + 12m + 6sm + 3st
H. umbellatum [3] ^∗	Southern Poland	UP	39.86	0.20	0.32	0.17	60.09	13.12	16.73	20.3	3.05	55.00	2x	18	10m + 8sm
H. racemosum subsp . barbatum [3] ^∗	Southern Poland	BP	40.78	0.18	0.31	0.16	58.88	11.41	16.63	18.4	2.52	68.10	3x	27	21m + 6sm
H. villosum [3] ^∗	Southern Poland	VP	38.93	0.22	0.34	0.23	61.26	16.26	22.80	22.1	3.14	112.95	4x	36	4M + 16m + 16sm
H. sabaudum [3] ^∗	Southern Poland	SP	38.9	0.22	0.34	0.20	60.99	18.86	20.11	22.1	3.13	84.60	3x	27	16m + 11sm
H. umbellatum [4] ^∗	Turkey, Trabzon	UT	41.65	0.17	0.28	0.15	58.47	4.55	14.73	16.7	3.03	54.62	2x	18	12 m + 6 m sat
[1] Selvi and Fiorini (1996), [2] Chrtek et al. (2004), [3] Szeląg and Vladimirov (2005), [4] Coşkuncelebi and Hayirlioğlu-Ayaz (2006).^∗Measurements and values were processed directly from the pictures of the cited papers.Index references – TF%: Huziwara (1962); As.k%: Arano and Saito (1980); A: Watanabe et al. (1999); A1 and A2: Romero Zarco (1986); CVCL and CVCI: (Paszko 2006); MCA: Peruzzi and Eroğlu (2013).

Figure 6 Scatter plots comparing the karyological parameters of some taxa belonging to Hieracium subgen. Hieracium, obtained (a) between A1 and A2 indices, and (b) between CV_CL and M_CA indices. For the meaning of the labels and index references, see Table 1.

Levan , A , Fredga , K and Sandberg , AA . 1964 . Nomenclature for centromeric position on chromosomes . Hereditas , 52 : 201 – 220 .

 Web of Science ®Google Scholar

Selvi , F and Fiorini , G . 1996 . Karyology of Hieracium L. subg. Hieracium (Asteraceae) from Mount Amiata (Central Italy) . Caryologia , 49 : 287 – 299 .

 Web of Science ®Google Scholar

Szeląg , Z and Vladimirov , V . 2005 . Chromosome numbers of Polish Hieracia (Asteraceae) . Polish Bot J , 50 ( 2 ) : 139 – 143 .

 Google Scholar

Coşkuncelebi , K and Hayirlioğlu-Ayaz , S . 2006 . Notes on chromosome numbers and karyotypes of five species in Hieracium L. s.str. (Asteraceae) from Turkey . Caryologia , 59 ( 1 ) : 19 – 24 .

 Google Scholar

Huziwara , Y . 1962 . Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosome of Aster . Am J Bot , 49 : 116 – 119 .

 Web of Science ®Google Scholar

Arano , H and Saito , H . 1980 . Cytological studies in family Umbelliferae 5. Karyotypes of seven species in subtribe Seselinae . Kromosomo , 2 : 471 – 480 .

 Google Scholar

Watanabe , K , Yahara , T , Denda , T and Kosuge , K . 1999 . Chromosomal evolution in the genus Brachyscome (Asteraceae, Astereae): Statistical tests regarding correlation between changes in karyotype and habit using phylogenetic information . J Plant Res , 112 : 145 – 161 .

 Web of Science ®Google Scholar

Paszko , A . 2006 . A critical review and a new proposal of karyotype asymmetry indices . Plant Syst Evol , 258 : 39 – 48 .

 Web of Science ®Google Scholar

Peruzzi , L and Eroğlu , HE . 2013 . Karyotype asymmetry: again, how to measure and what to measure? . Comp Cytogenet , 7 ( 1 ) : 1 – 9 .

 PubMed Web of Science ®Google Scholar