Parental origin and genomic evolution of tetraploid Gossypium species by molecular marker and GISH analyses

Figures & data

Table 1. The details of cotton accessions used in the experiment.

No.	Material	Genome
1	G. arboreum L.	A2
2	G. herbaceum L.	A1
3	G.aridum (Rose & Standl.) Skov.	D4
4	G. thurberi Tod.	D1
5	G. klotzschianum Anderss.	D3-k
6	G. davidsonii Kell.	D3-d
7	G. trilobum (Moc. & Sess. ex DC.) Skov.	D9
8	G. gossypioides Ulbr.	D6
9	G. raimondii Ulbr.	D5
10	G. hirsutum L.	(AD)1
11	G. barbadense L.	(AD)2
12	G. mustillinum Miers & Watt.	(AD)4
13	G. darwinii Watt.	(AD)5

Figure 1. Dendrogram of cotton accessions based on RAPD and SSR markers.

Table 2. The similarity coefficient result between tetraploid species and A- and D-genome species by RAPD and SSR markers.

Material	Genetic similarity coefficients
	G.herbaceum A1	G.arboreum A2	G.aridum D4	G.thurberi D1	G.klotzschianum D3-k	G.davidsonii D3-D	G.trilobum D9	G.gossypioides D6	G.raimondii D5
G.hirsutum	0.732	0.738	0.661	0.705	0.721	0.699	0.683	0.732	0.776
G.barbadense	0.705	0.670	0.656	0.667	0.705	0.694	0.645	0.716	0.770
G.mustillinum	0.721	0.727	0.628	0.661	0.689	0.678	0.628	0.721	0.765
G. darwinii	0.727	0.732	0.645	0.689	0.694	0.683	0.656	0.727	0.760
Mean	0.721	0.717	0.648	0.681	0.702	0.689	0.653	0.724	0.768

Figure 2-7 (Color online) GISH photographs of tetraploid cotton species (G. hirsutum and G. barbadense) with some diploid cotton species (G. arboreum, G. herbaceum and G. raimondii). (2) Target chromosomes of G. hirsutum hybridized with G. herbaceum as a probe and G. raimondii as a block (red fluorescent signals indicated those hybridized with probe and blue ones were the block, the same in other photographs). (3) Target chromosomes of G. hirsutum hybridized with G. arboreum as a probe and G. raimondii as a block. (4) Target chromosomes of G. barbadense hybridized with G. arboreum as a probe and G. raimondii as a block. (5) Target chromosomes of G. barbadense hybridized with G. raimondii as a probe and G. herbaceum as a block. (6) Target chromosomes of G. hirsutum hybridized with G. raimondii as a probe and G. herbaceum as a block. (7) Target chromosomes of G. hirsutum hybridized with G. raimondii as a probe and G. arboreum as a block. Bars in all figures = 5 μm.