Karyotype analysis of Indian lentils through EMA based Giemsa staining

Figures & data

Table 1. List of the Lens accessions studied with their source and germination efficiency.

Serial no.	Lens species and cultivars/germplasms	Figure	Source	Germination (%)
1	Lens odemensis IIWL 35	Figure 1A	Indian Institute of Pulse Research, Kanpur, India	80
2	Lens orientalis IIWL 248	Figure 1B	Do	80
3	Lens culinaris IPL 81	Figure 1C	Do	78.45
4	Lens culinaris IPL 316	Figure 1D	Do	53.58
5	Lens culinaris Jl-1	Figure 1E	Do	75.25
6	Lens culinaris PL 1406	Figure 1F	Do	65.56
7	Lens culinaris KLS 210	Figure 1G	Do	36.36
8	Lens culinaris EC 70403 (NBPGR)	Figure 1H	Do	88.65
9	Lens culinaris EC 78455 (NBPGR)	Figure 1I	Do	84.62
10	Lens culinaris EC 78498 (NBPGR)	Figure 2A	Do	94.73
11	Lens culinaris EC 267877 (NBPGR)	Figure 2B	Do	94.12
12	Lens culinaris micro type	Figure 2C	Bankura, West Bengal	97.14
13	Lens culinaris macro type	Figure 2D	Barasat, West Bengal	80
14	Lens culinaris micro type	Figure 2E	Barasat, West Bengal	60.25

Figure 1. Giemsa stained well-scattered somatic metaphase plates of nine Lens species and cultivars (2n =14): (A) L. odemensis (ILWL 35); (B) L. orientalis (ILWL 248); (C) L. culinaris (IPL81); (D) L. culinaris (IPL316); (E) L. culinaris (JL-1); (F) L. culinaris (Pl406); (G) L. culinaris (KLS 210); (H) L. culinaris (EC704030); (I) L. culinaris (EC78455). Scale bars: 5 μm.

Figure 2. Giemsa and orcein stained well-scattered somatic metaphase plates in different cultivars of L. culinaris: (A) L. culinaris (EC 78498); (B) L. culinaris (EC267877); (C) L. culinaris (micro, Bankura); (D) L. culinaris (macro, Barasat); (E) L. culinaris (micro, Barasat); (F) monosomic metaphase plate of L. culinaris (EC, 78410) showing 2n = 13 chromosomes; (G) monosomic metaphase plate of L. culinaris (IPL81) showing 2n = 26 almost separated chromatids; (H) L. culinaris ((IPL316) metaphase plate showing 2n = 28 almost separated chromatids; (I) orcein stained somatic metaphase plate with 2n = 14 chromosomes in L. culinaris (macro Barasat). Scale bars: 5 μm.

Table 2. Comparative karyometric analysis of 14 Lens species and cultivars.

Serial no.	Species	Cultivar/germplasm,with symbol in figures	Length of long chromosome (μm) (mean ± SE)	Length of small chromosome (μm) (mean ± SE)	No. of satellite chromosomes	Satellite chromosomes in pair	Diploid chromatin length (μm) (mean ± SE)	Haploid karyotype formula
1	Lens odemensis	ILWL-35 [A]	8.0 ± 0.05^a	5.6 ± 0.02^b	1 pair	4	94.8 ± 0.05^a	3m + 1m (sat) + 2Sm + 1St
2	L. orientalis	ILWL-248 [B]	7.2 ± 0.07^b	5.4 ± 0.05^a	1 pair	4	84.0 ± 0.06^a	3m + 1m (sat) + 2Sm + 1St
3	Lens culinaris	IPL 81 [C]	8.0 ± 0.05^a	5.6 ± 0.01^b	1 pair	4	89.6 ± 0.06^a	3m + 1m(sat) + 2Sm + 1St
4	L. culinaris	IPL 316 [D]	8.4 ± 0.04^a	5.6 ± 0.01^b	1 pair	3	96.0 ± 0.03^a	3m + 1m(sat) + 2Sm + 1St
5	L. culinaris	JL-1 [E]	9.2 ± 0.06^a	6.4 ± 0.04^a	1 pair	3	109.6 ± 0.01^b	3m + 1m(sat) + 2Sm + 1St
6	L. culinaris	PL 1 406 [F]	9.2 ± 0.06^a	6.0 ± 0.04^a	1 pair	4	104.4 ± 0.01^b	3m + 1m (sat) + 2Sm + 1St
7	L. culinaris	KLS 210 [G]	10.4 ± 0.12^b	7.2 ± 0.06^a	1 pair	4	121.2 ± 0.12^b	3m + 1m (sat) + 2Sm + 1St
8	L. culinaris	EC 70403(NBPGR) [H]	8.8 ± 0.1^b	6.4 ± 0.03^b	1 pair	4	107.6 ± 0.04 ^a	3m + 1m (sat) + 2Sm + 1St
9	L. culinaris	EC 78455 (NBPGR) [I]	8.8 ± 0.11^b	6.0 ± 0.01^b	1 pair	4	104 ± 0.01^c	3m + 1m (sat) + 2Sm + 1St
10	L. culinaris	EC 78498 (NBPGR) [J]	5.2 ± 0.06^a	3.6 ± 0.12^c	1 pair	3	70.8 ± 0.04^a	3m + 1m (sat) + 2Sm + 1St
11	L. culinaris	EC 267877(NBPGR)[K]	7.6 ± 0.05^a	5.1 ± 0.09^a	1 pair	4	89.0 ± 0.05^a	3m + 1m (sat) + 2Sm + 1St
12	L. culinaris	Micro (Bankura) [L]	9.2 ± 0.06^a	6.0 ± 0.11^a	1 pair	4	106.4 ± 0.06^a	3m + 1m (sat) + 2Sm + 1St
13	L. culinaris	Macro (Barasat) [M]	4.4 ± 0.02^c	3.2 ± 0.06^a	1 pair	3	53.6 ± 0.06^a	3m + 1m (sat) + 2Sm + 1St
14	L. culinaris	Micro (Barasat) [N]	8.8 ± 0.09^b	5.6 ± 0.01^b	1 pair	3	100.8 ± 0.01^c	3m + 1m (sat) + 2Sm + 1St

Mean values having different letters in superscript are significantly different from each other (p ≤ 0.05) according to Duncan’s multiple range test (DMRT) (Wang et al. 2009).

Figure 3. Hand drawings of Lens species and cultivars showing position of one pair interstitial sat chromosomes: (A) L. odemensis (ILWL 35); (B) L. orientalis (ILWL 248); (C) L. culinaris (IPL81); (D) L. culinaris (IPL316); (E) L. culinaris (JL-1); (F) L. culinaris (Pl406); (G) L. culinaris (KLS 210); (H) L. culinaris (EC704030); (I) L. culinaris (EC78455); (J) L. culinaris (EC 78498); (K) L. culinaris (EC267877); (L) L. culinaris (micro, Bankura); (M) L. culinaris (macro, Barasat); (N) L. culinaris (micro, Barasat). Scale bars: 5 μm.

Figure 4. Idiograms of 14 species and cultivars of Lens. Chromosomes are arranged in order of descending length: (A) L. odemensis (ILWL 35); (B) L. orientalis (ILWL 248); (C) L. culinaris (IPL81); (D) L. culinaris (IPL316); (E) L. culinaris (JL-1); (F) L. culinaris (Pl406); (G) L. culinaris (KLS 210); (H) L. culinaris (EC704030); (I) L. culinaris (EC78455); (J) L. culinaris (EC 78498); (K) L. culinaris (K. EC2678); (L) L. culinaris (micro, Bankura); (M) L. culinaris (macro, Barasat); (N) L. culinaris (micro, Barasat). Scale bars: 2 μm.

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