Monitoring the introgression of E genome chromosomes into triticale using multicolor GISH

Figures & data

Table 1. McGISH analysis of segregating populations derived from triticale T182 (ABR) × partial amphiploid 8801(ABE).

Generation	F2				F3				F4				F5				F6
Line	Ch	R	E	Tr	Ch	R	E	Tr	Ch	R	E	Tr	Ch	R	E	Tr	Ch	R	E	Tr
1	42	7	7		36–42	5–9	7–8	1–3E/R	40–43	3–4	2–5	-
3	42	8	6		42	11	2	2E/R	38–40	10–12	0–1	0–1	42	14
4a					42–46	4–11	5–9	1–2E/R	30–45	1–10	2–10	1–3	42	3	10	1	36	2	8	1E/R+1W/R
4b									41–43	10–14	1–3		41–42	12–14	1–3		42	12	2
5	42	7	7		32–38	1–9	6–10	1	29–38	1–7	1–9	0–1	28
6					28–29	0–1	0–1	0–1	28–29	0–1	0–1		28

Abbreviations: Ch, total chromosome number; R, number of R genome chromosomes; E, number of E genome chromosomes; Tr, number of E/R translocations; W/R, translocations between wheat and R genome chromosomes.

Figure 1. An F₆ mitotic cell of line 4b showing a total of 42 chromosomes consisting of 28 wheat plus 12 R genome chromosomes (green) and 2 E genome chromosomes (red).

Figure 2. E/R translocated chromosomes: (a) An F₄ cell of line 5 with 28 chromosomes including one R (green) genome chromosome plus an E/R translocated chromosome (arrowed); (b) an F₄ cell of line 4a showing four E genome chromosomes, 10 plus two telo R genome chromosomes plus two E/R translocated chromosomes (arrowed); (c) an F₄ cell of line 4a showing eight E genome chromosomes, seven R genome chromosomes plus two E/R translocated chromosomes (arrowed); (d) an F₄ cell of line 4a showing 10 E genome chromosomes, five plus telo R genome chromosomes plus two E/R translocated chromosomes (arrowed).

Figure 3. An F₄ cell of line 4A showing eight E genome chromosomes, two from the R genome plus one E/R and one wheat/R translocated chromosomes (arrowed).

Table 2. Meiotic characteristics of F₄ and F₅ progenies of line 4 derived from intercrosses of triticale T182 (ABR) × partial amphiploid 8801(ABE).

		F4	F5
I		1.8 (0–4)	1.3 (0–4)
II	Ring	9.5 (6–14)	9.1 (4–14)
	Rod	10.6 (7–12)	11.3 (8–16)
Xta		29.6	29.4

Abbreviation: Xta, average chiasmata frequency per cell.