Comparative cytogenetics of two sympatric species (Gymnocypris, Schizothoracinae) from the Lake Langcuo, Qinghai-Tibet Plateau

Figures & data

Table 1. A summary of the cytogenetic data available for the Schizothoracinae.

Species	2n	Karyotypic formula	Locality	Reference
Chuanchia labiosa	92	32m+26sm+18st+16t	Yellow River, China	Yu et al. 1990
Diptychus sp.	98	28m+32sm+38st-t	Lake Fuxian, China	Zan et al. 1985
Diptychus maculatus	100	70m/sm+30st/a	Kirghiz Tan	Mazik et al. 1989
Diptychus sewerzowi	98	80m/sm +18st/a	Kirghiz Tan	Mazik et al. 1989
Gymnodiptychus dybowskii	98	28m+30sm+12st+28t	Manasi River, China	Kong et al. 2011
Gymnodiptychus pachycheilus	90–98	—	—	Collares-Pereira, 1994
Gymnocypris dobula	66	32m+10sm+4st+20t	Tingri County, China	Wu et al. 1999
Gymnocypris eckloni	94	26m+28sm+22st+18t	Yellow River, China	Yu et al. 1990
Gymnocypris przewalskii	92	18m+18sm+16st+40t	Lake Qinghai, China	Qi 2004
	92	32m+22sm+24st+14t	Lake Qinghai, China	Yan et al. 2007
Gymnocypris waddelli	94	24m+14sm+22st+34t	Pumqu River, China	Wu et al. 1999
Oxygymnocypris stewartii	92	26m+30sm+22st+14t	Lasha River, China	Yu et al. 1990
	86	24m+12sm+12st+18t	Nianchu River, China	Wu et al. 1999
Platypharodon extremus	90	24m+30sm+20st+16t	Yellow River, China	Yu et al. 1990
Ptychobarbus dipogon	424–432	—	Lasha River, China	Wu et al. 1999
	417–470	—	—	Yu and Yu 1990
Schizopygopsis pylzovi	92	32m+26sm+20st+14t	Yellow River, China	Yu et al. 1990
	92	24m+24sm+22st+22t	Yellow River, China	Chen et al. 2006
Schizopygopsis younghusbandi younghusbandi	90	26m+28sm+20st+16t	Lasha River, China	Yu et al. 1990
Schizopygopsis younghusbandi himalayensis	88	40m+16sm+12st+20t	—	Wu et al. 1999
Schizothorax sp.	148	50m+28sm+70st-t	ErYuan County, China	Zan et al. 1985
Schizothorax curvifrons	94	26m+20sm+20st+28t	Sindh stream, India	Ganai et al. 2011, 2012, 2014
Schizothorax daliensis	148	48m+30sm+70st-t	Erhai, China	Zan et al. 1985
Schizothorax davidi	98	20m+34sm+24st+20t	Qingyi River, China	Li et al. 1987
Schizothorax esocinus	98	30m+22sm+10st+36t	Lake Dal, India	Ganai et al. 2011, 2014
Schizothorax grahami	148	52m+30sm+66st-t	Luquan County, China	Zan et al. 1985
Schizothorax kozlovi	96	36m+16sm+10st+34t	Wujiang River, China	Chen 2013
	128	26m+22sm+24st+56t	Wujiang River, China	Zou 2009
Schizothorax kumaonensis	98	24m+6sm+68st-a	—	Lakara et al. 1997
Schizothorax labiatus	98	24m+20sm+2st+52t	Lake Dal, India	Ganai et al. 2011, 2014
Schizothorax lissolabiatus	148	38m+32sm+78st/a	Yunan, China	Zan et al. 1986
Schizothorax macropogon	90–98	—	—	Collares-Pereira, 1994
	102	20m+28sm+22st+16t	Lasha River, China	Wu et al. 1999
Schizothorax niger	98	24m+32sm+22st+20t	Lake Dal, India	Ganai et al. 2011, 2012, 2014
	98	22m+26sm+50st-t	India	Khuda-Bukhsh and Nayak 1982
Schizothorax oconnori	92	30m+26sm+20st+16t	Lasha River, China	Yu et al. 1990
	106	24m+26sm+30st+25t	Nianchu River, China	Wu et al. 1999
Schizothorax plagiostomus	96	24m+18sm+54t	Jhelum River, India	Ganai et al. 2011, 2014
Schizothorax prenanti	148	28m+40sm+36st+44t	Qingyi River, China	Li et al. 1987
Schizothorax progastus	98	16m+20sm+16st+40t	Haryana, India	Rishi et al. 1983
Schizothorax richardsonii	98	24m+28sm+24st+22a	—	Lakara et al. 1997
Schizothorax taliensis	148	48m+28sm+72st/a	Lake Erhai, China	Zan et al. 1985
Schizothorax waltoni	92	30m+26sm+20st+16t	Lasha River, China	Yu et al. 1990
	112	26m+24sm+28st+34t	Nianchu River, China	Wu et al. 1999
Schizothorax yunnanensis	148	—	—	Collares-Pereira, 1994
Schizothorax zarudnyi	96	9m + 14sm + 25st	Hamoon Lake, Iran	Kalbassi et al. 2008

Figure 1. Giemsa stained karyotypes of Gymnocypris chui. m: metacentric; sm: submetacentric; st: subtelocentric; a: acrocentric. The bar represents 10 μm.

Figure 2. Giemsa stained karyotypes of Gymnocypris scleracanthus. m: metacentric; sm: submetacentric; st: subtelocentric; a: acrocentric. The bar represents 10 μm.

Figure 3. Physical localization of ribosomal genes on metaphase chromosomes in (a–c) Gymnocypris chui: (a) fluorescent in situ hybridization with 45S rDNA probe; (b) metaphase showing DAPI staining; and (c) sites of 45S rDNA; and (d–f) G. scleracanthus: (d) fluorescent in situ hybridization with 45S rDNA probe; (e) metaphase showing DAPI staining; (f) sites of 45S rDNA.

Yu X, Li Y, Zhou T. 1990. Karyotype studies of cyprinid fishes in China-comparative study of the karyotypes of 8 species of schizothoracine fishes. J Wuhan Univ. (2):97–104.

 Google Scholar

Zan R, Liu W, Song Z. 1985. Tetraploid-hexaploid relationship in schizothoracinae. Acta Genet Sin. 12(2):137–142.

 Google Scholar

Mazik EJ, Toktosunov A, Ráb P. 1989. Karyotype study of four species of the genus Diptychus (Pisces, Cyprinidae), with remarks on polyploidy of schizothoracine fishes. Folia zool. 38(4):325–332.

 Web of Science ®Google Scholar

Kong L, Hu W, Wang J. 2011. Primary study on karyotype and C-banding of Gymnodiptychus dybowskii kessler. J Biol. 28(1):34–36.

 Google Scholar

Collares-Pereira MJ. 1994. The karyology of barbins and the possible pleisiomorphic condition of polyploidy in Cyprinidae. Bull Fr Pêche Pisc. 67(334):191–199.

 Google Scholar

Wu Y, Kang B, Men Q, Wu C. 1999. Chromosome diversity of Tibetan fishes. Zool Res. 20(4):258–264.

 Google Scholar

Qi D. 2004. Preliminary studies on chromosome karyotype and polyploidy of Qinghai-lake naked carp. J Qinghai Univ. 22(2):44–47.

 Google Scholar

Yan X, Shi J, Sun X, Liang L. 2007. Study on the karyotype of Gymnocypris przewalskii. J Northeast Agr Univ. 38(5):645–648.

 Google Scholar

Yu X, Yu X. 1990. A schizothoracine fish species, Diptychus dipogon, with a very high number of chromosomes. Chromosome Inf Serv. (48):17–18.

 Google Scholar

Chen YQ, Yang C, Zhao J, Li KM, Tang WJ, Wang ZJ. 2006. The chromosome karyotype of Yellow River Schizothoracin (Schizopygopsis pylzovi). Fisheries Sci. 25(11):577–580.

 Google Scholar

Ganai FA, Yousuf AR, Dar SA, Tripathi NK, Wani SU. 2011. Cytotaxonomic status of schizothoracine fishes of Kashmir Himalaya (Teleostei: Cyprinidae). Caryologia. 64(4):435–445.

 Web of Science ®Google Scholar

Ganai FA, Dar SA, Yousuf AR, Tripathi NK, Wani S. 2012. Karyoevolutionary and karyosystematic considerations on Schizothorax curvifrons and Schizothorax niger (Teleostei: Cyprinidae): Important hill-stream food fishes of Kashmir Himalaya. Afr J Biotechnol. 11(57):11,998–12,004.

 Google Scholar

Ganai FA, Wani S, Ahmad S, Yousuf AR, Tripathi NK. 2014. Coupled biochemical genetic and karyomorphological analyses for taxonomic classification-A case study of Schizothorax species complex (Teleostei: Cyprinidae). Afr J Biotechnol. 13(15):1623–1630.

 Google Scholar

Li Y, Li K, Gui J, Zhou D. 1987. Studies on the karyotypes of Chinese cyprinid fishes XI. karyotypes of two species of schizothoracinae and three species of gobiobotinae. Acta Hydrobiol Sin. 11(2):184–186.

 Google Scholar

Chen YX. 2013. The genetic characterization and population genetic diversity of Schizothorax kozlovi (Nikolsky) [PhD thesis]. [Ya'an (China)]: Sichuan Agricultural University.

 Google Scholar

Zou XJ. 2009. Study on karyotype and genetic diversity in the population of Schizothorax (Racoma) kozlovi [Master's thesis]. [Guiyang (China)]: Guizhou University.

 Google Scholar

Lakara WS, John G, Barat A. 1997. Cytogenetic studies on endangered and threatened fishes 2. Karyotypes of two species of snow-trout, Schizothorax richardsonii (Gray) and S. kumaonensis (Menon). Proc Natl Acad Sci India Biol Sci. 67(1):79–81.

 Google Scholar

Zan RG, Song Z, Liu WG. 1986. Studies on karyotypes and nuclear DNA contents of some cyprinid fishes, with notes on fish polyploids in China. In: Uyeno T, Arai R, Taniuchi T, Matsuura K, editors. Indo-Pacific fish biology. Tokyo: Ichthyological Society of Japan; p. 887–885.

 Google Scholar

Khuda-Bukhsh AR, Nayak K. 1982. Karyomorpological studies in two species of hill stream fishes from Kashmir, India: occurrence of a high number of chromosomes. Chromosome Inf Serv. 33:12–14.

 Google Scholar

Rishi KK, Singh J, Kaul MM. 1983. Chromosomal analysis of Schizothoraichthys progastus (McClell.) (Cyprinidae: Cypriniformes). Chromosome Inf Serv. 34:12–13.

 Google Scholar

Kalbassi MRK, Hosseini SV, Tahergorabi R. 2008. Karyotype analysis in Schizothorax zarudnyi from Hamoon lake. Iran. Turk J Fish Aquat S. 8(2):335–340.

 Web of Science ®Google Scholar