DNA amount of chicken chromosomes resolved by image cytometry

Figures & data

Table 1. 1C DNA amount of chicken chromosomes.

Group^a	Chromosome number	1C in pg (Bloom et al., 1993)	1C in pg (Smith and Burt 1998)	1C in bp × 10^9 (Kersey et al., 2015)^b	Mean 1C in pg (present study)^b	Mean 1C in bp × 10^9 (present study)^b
A	1	0.2515	0.2556	0.1997	0.158	0.1545
A	2	0.1575	0.1851	0.1522	0.121	0.1183
A	3	0.1104	0.1411	0.1129	0.093	0.0910
A	4	0.0869	0.1115	0.0922	0.077	0.0753
A	5	0.0716	0.0654	0.0609	0.052	0.0509
A	6	0.0389	0.0429	0.0357	0.041	0.0401
A	7	0.0389	0.0419	0.0371	0.036	0.0352
A	8	0.0235	0.0307	0.0294	0.034	0.0333
A	9	0.0235		0.0240	0.030	0.0293
A	10			0.0204	0.028	0.0274
B	11			0.0198	0.026	0.0254
B	12			0.0203	0.025	0.0245
B	13			0.0182	0.023	0.0225
B	14			0.0155	0.021	0.0205
B	15			0.0129	0.021	0.0205
B	16	0.0164		0.0001	0.020	0.0196
C	17			0.0107	0.018	0.0176
C	18			0.0115	0.017	0.0166
C	19	0.0153		0.0102	0.015	0.0147
C	20			0.0146	0.015	0.0147
C	21			0.0070	0.013	0.0127
C	22			0.0042	0.013	0.0127
C	23			0.0059	0.011	0.0108
C	24			0.0065	0.011	0.0108
C	25			0.0022	0.011	0.0108
C	26			0.0054	0.011	0.0108
C	27			0.0053	0.010	0.0098
C	28			0.0048	0.010	0.0098
C	29	0.0102			0.009	0.0088
C	30				0.008	0.0078
C	31–32				0.007	0.0068
D	33–34				0.006	0.0059
D	35–37				0.004	0.0039
D	38				0.003	0.0029
A	Z	0.3384	0.1033	0.0842	0.041	0.0401
A	W	0.0235	0.0348	0.0013	0.014	0.0137

^a The chromosome group is based on the chicken karyotype classification of Masabanda et al. (2004).

^b 1C mean values converted to base pairs (bp), considering that 1 pg of DNA corresponds to 0.978 × 10⁹ bp (Doležel et al. 2003).

Figure 1. Relationship between the DNA mean values of each male (axis x) and female (axis y) chicken chromosomes measured from ICM. For Pearson linear correlation and regression analysis, the mean values of DNA amount were multiplied by 1000.

Figure 2. Representative karyogram of (a) the female and (b) the male chicken assembled from metaphasic chromosomes stained from Feulgen reaction. The criteria established in the 4th European Colloquium of Cytogenetics of Domestic Animals and the chromosomal DNA amount were considered to consecutively order and number the chromosomes. The karyograms showed 76 autosomal and ZZ (male) or ZW (female) sexual chromosomes. Bar = 5 μm.

Bloom SE, Delany ME, Muscarella DE. 1993. Constant and variable features of avian chromosomes. In: Etches RJ, Gibbon AMV, editors. Manipulation of the avian genome. 1st ed. Boca Raton: CRC Press; p. 39–59.

 Google Scholar

Smith J, Burt DW. 1998. Parameters of the chicken genome (Gallus gallus). Anim Genet. 29(4):290–294. doi:10.1046/j.1365-2052.1998.00334.x.

 PubMed Web of Science ®Google Scholar

Kersey PJ, Allen JE, Armean I, Boddu S, Bolt BJ, Carvalho-Silva D, Christensen M, Davis P, Falin LJ, Grabmueller C, et al. 2015. Ensembl Genomes 2016: more genomes, more complexity (Release 83). Nucleic Acids Res. 44(D1):D574–D580. doi:10.1093/nar/gkv1209.

 PubMed Web of Science ®Google Scholar

Masabanda JS, Burt DW, O’Brien PCM, Vignal A, Fillon V, Walsh PS, Cox H, Tempest HG, Smith J, Habermann F, et al. 2004. Molecular cytogenetic definition of the chicken genome: the first complete avian karyotype. Genetics. 166(3):1367–1373. doi:10.1534/genetics.166.3.1367.

 PubMed Web of Science ®Google Scholar