Cytogenetic studies on six species of the leuciscine genus Pseudophoxinus Bleeker, 1860 (Teleostei, Cyprinidae)

Figures & data

Table 1. General information regarding collected specimens.

Species	Locality	Coordinates	Date	Number and sex of specimens
P. battalgilae	Kuğulupark, Seydişehir, Konya	37°23′N, 31°50′E	2013	3 ♀, 8 ♂
P. burduricus	Düğer Village, Burdur	37°34′N, 30°01′E	2012	4 ♀, 6 ♂
P. egridiri	Eğirdir Lake, Isparta	38°07′N, 30°54′E	2012	1 ♀, 7 ♂
P. evliyae	Kırkpınar Village, Korkuteli, Antalya	37°08′N, 29°55′E	2013	11 ♀, 10 ♂
P. fahrettini	Köprüçay River, Pazarköy, Isparta	37°44′N, 31°01′E	2012	1 ♀, 5 ♂
P. maeandri	Işıklı Spring, Çivril, Denizli	38°19′N, 29°51′E	2013	4 ♀, 3 ♂

Figure 1. Giemsa stained metaphases and arranged karyotypes of (a) P. battalgilae; (b) P. burduricus; (c) P. egridiri; (d) P. evliyae; (e) P. fahrettini; (f) P. maeandri. Scale bar = 5 μm.

Figure 2. C-banded metaphases of (a) P. battalgilae; (b) P. burduricus; (c) P. egridiri; (d) P. evliyae; (e) P. fahrettini; (f) P. maeandri. Scale bar = 5 μm.

Figure 3. Ag-stained metaphases of (a) P. battalgilae; (b) P. burduricus; (c) P. egridiri; (d) P. evliyae; (e) P. fahrettini; (f) P. maeandri. Arrows indicate the NORs. Scale bar = 5 μm.

Table 2. NOR number polymorphism of the four studied species.

Species	Number of Ag-stained metaphases	Ag-positive signals (%)
		1	2	3	4	5	6
P. battalgilae	55	3.63	32.72	23.63	38.18	1.84
P. burduricus	65	32.30	53.84	9.23	4.63
P. evliyae	121		10.74	38.84	40.49	7.43	2.50
P. fahrettini	46	2.17	89.13	1.69	7.01

Figure 4. Ag-stained metaphases with number and/or location polymorphisms of (a, b) P. battalgilae showing two and three Ag-positive signals; (c, d) P. burduricus showing one and three Ag-positive signals; (e–j) P. evliyae showing from two to six Ag-positive signals; (k, l) P. fahrettini showing two and three Ag-positive signals; (m) P. fahrettini with size polymorphism. Arrows indicate the NORs. Scale bar = 5 μm.

Table 3. Karyological studies in the genus Pseudophoxinus.

Species	2n	Karyotype	FN	Reference
P. antalyae	50	16m+14sm+12st+8a	92	Ergene et al. 2010
P. firati	50	38m-sm+12st	88	Karasu et al. 2011
P. elizavetae	50	8m+34sm+8st	92	Gaffaroğlu et al. 2014
P. crassus	50	12m+30sm+8st-a	92	Ünal et al. 2014
P. hittitorum	50	14m+26sm+10st-a	90	Ünal et al. 2014
P. zekayi	50	16m+26sm+8st-a	92	Ünal 2015
P. battalgilae	50	16m+28sm+6st-a	94	Current study
P. burduricus	50	18m+26sm+6st-a	94	Current study
P. egridiri	50	14m+28sm+8st-a	92	Current study
P. evliyae	50	14m+30sm+6st-a	94	Current study
P. fahrettini	50	16m+26sm+8st-a	92	Current study
P. maeandri	50	10m+32sm+8st-a	92	Current study

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Karasu M, Yüksel E, Gaffaroğlu M. 2011. Karyotype, NORs, and C-banding analysis of Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae) in the Euphrates River. Turkey. Turk J Zool. 35(6):865–868.

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