First cytogenetic characterization of Loricaria simillima (Loricariidae, Siluriformes) from Paraná River (Argentina) with emphasis in cytotaxonomy of Loricaria

Figures & data

Figure 1. Giemsa stained karyotype of Loricaria simillima. The boxes show Ag-NOR, C-banding, CMA₃ and DAPI staining of the eighth pair.

Figure 2. Loricaria simillima: (a) conventional Giemsa staining; arrows indicate the eighth pair. (b) C-banding technique; arrows indicate the NOR bearing pair and an acrocentric chromosome with conspicuous interstitial band. (c) Silver staining; arrows indicate the NOR bearing pair.

Figure 3. Loricaria simillima sequential fluorescent staining with: (a) CMA₃ and (b) DAPI. Arrows indicate the NOR bearing pair.

Table 1. Cytogenetic studies in genus Loricaria.

Species	Locality	2n	Karyotypic formulae	NORs	Reference
Loricaria sp.	Parana river, PR	64	10 m+6sm+ 4st+44a (B)	10p	1
L. carinata	Parana river, ARG	64	12 m-sm + 52st-a	–	2
L. cataphracta	Onça stream, MS	64	12 m+8sm+2st+42a	12q (IN)	3
	Onça stream, MS	64	12 m+8sm+2st+42a	8q (IN)	3
	Onça stream, MS	64	12 m+8sm+2st+42a	8q; 12q (IN)	3
	Onça stream, MS	64	12 m+8sm+2st+42a	9p;12q (IN)	3
	Onça stream, MS	64	12 m+8sm+2st+42a	12q; 13q (IN)	3
L. simillima	Parana river, ARG	64	12 m+12sm+40st/a	8p (IN)	4

2n: diploid number; NORs: nucleolus organizer regions; PR: Parana; MS: Mato Grosso do Sul; SP: Sao Paulo; ARG: Argentina; m: metacentric; sm: sub-metacentric; st: sub-telocentric; a: acrocentric; IN: interstitial; (B): supernumerary chromosomes; p: short arm; q: long arm.

References: (1) Scavone and Ferreira-Julio (1994); (2) Fenocchio et al. (2003); (3) Porto et al. (2014a); (4) this study.

Scavone, MDP, Ferreira-Julio, H. 1994. Cytogenetics analysis and probable supernumerary chromosomes of Loricaria prolixa and Loricaria sp. females (Loricariidae-Siluriformes) from the Paraná River basin. Rev. de Ictiología 2/3(1/2); pp. 41–47.

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Porto FE, Gindri BS, Vieira MMR, Borin LA, Portela-Castro ALB, Martins-Santos IC. 2014a. Polymorphisms of the nucleolus organizing regions in Loricaria cataphracta (Siluriformes, Loricariidae) of the upper Paraguay River basin indicate an association with transposable elements. Genet Mol Res. 13(1):1627–1634. doi:10.4238/2014.March.12.15.

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