Genetic diversity study of Brassica campestris L. ssp. chinensis Makino based on ISSR markers

Figures & data

Table 1. The accessions of Brassica campestris L. ssp. chinensis Makino from different countries investigated in this study.

No.	Accession name	Origin	Fertility	Varity type	No.	Accession name	Origin	Fertility	Varity type
1	AikangqingA	China	Sterile	var. communis Tsen et Lee	35	Wuyueman	China	Fertile	var. communis Tsen et Lee
2	Aikangqing	China	Fertile	var. communis Tsen et Lee	36	Shanhaiqing	China	Fertile	var. communis Tsen et Lee
3	Tiannenghuangjin	China	Fertile	var. communis Tsen et Lee	37	Huashusijiqing	China	Fertile	var. communis Tsen et Lee
4	NaibaicaiA	China	Sterile	var. communis Tsen et Lee	38	Teaiqing	China	Fertile	var. communis Tsen et Lee
5	CK5–2	China	Fertile	var. communis Tsen et Lee	39	Siyueman	China	Fertile	var. communis Tsen et Lee
6	Heixinwu	China	Fertile	var. communis Tsen et Lee	40	YakangA	China	Sterile	var. communis Tsen et Lee
7	Zaoshubaicaitai	China	Fertile	var. communis Tsen et Lee	41	Zhengwangda	China	Fertile	var. communis Tsen et Lee
8	AibaigengA	China	Sterile	var. communis Tsen et Lee	42	Zhongqiqing	China	Fertile	var. communis Tsen et Lee
9	Jiangyibaiguan	China	Fertile	var. communis Tsen et Lee	43	Suzhouqing	China	Fertile	var. communis Tsen et Lee
10	Aibaigeng	China	Fertile	var. communis Tsen et Lee	44	Naibaicai	China	Fertile	var. communis Tsen et Lee
11	Aijiaohuang	China	Fertile	var. communis Tsen et Lee	45	Lixiangqing	China	Fertile	var. communis Tsen et Lee
12	Baimeiren	China	Fertile	var. communis Tsen et Lee	46	Heixinwu	China	Fertile	var. rosularis Tsen et Lee
13	Tihuwei	China	Fertile	var. communis Tsen et Lee	47	Baiyetacai	China	Fertile	var. rosularis Tsen et Lee
14	AizibaiA	China	Sterile	var. communis Tsen et Lee	48	ZaoshuNo5	China	Fertile	var. communis Tsen et Lee
15	Hualiangyu	China	Fertile	var. communis Tsen et Lee	49	Yakang	China	Fertile	var. communis Tsen et Lee
16	Shanghainaiyou	China	Fertile	var. communis Tsen et Lee	50	Naire605	China	Fertile	var. communis Tsen et Lee
17	Baimeigui	China	Fertile	var. communis Tsen et Lee	51	Lidun	China	Fertile	var. communis Tsen et Lee
18	Huaguan	Japan	Fertile	var. communis Tsen et Lee	52	QinggengP0410	Japan	Fertile	var. communis Tsen et Lee
19	Zaoshenhuajing	Japan	Fertile	var. communis Tsen et Lee	53	Shuanshanxiaobaicai	Japan	Fertile	var. communis Tsen et Lee
20	Jinxia	Japan	Fertile	var. communis Tsen et Lee	54	Xiadi	Japan	Fertile	var. communis Tsen et Lee
21	P0405	Japan	Fertile	var. communis Tsen et Lee	55	P0506	Japan	Fertile	var. communis Tsen et Lee
22	P0411	Japan	Fertile	var. communis Tsen et Lee	56	Xiashang	Japan	Fertile	var. communis Tsen et Lee
23	Taiguozhongzi No.1	Thailand	Fertile	var. communis Tsen et Lee	57	Taiguozhongzi No.3	Thailand	Fertile	var. communis Tsen et Lee
24	Sijiqing	China	Fertile	var. communis Tsen et Lee	58	Qingxiangcai	China	Fertile	var. communis Tsen et Lee
25	Jinyinfeng A	China	Sterile	var. communis Tsen et Lee	59	AijiaohuangA	China	Sterile	var. communis Tsen et Lee
26	Jinyinfeng	China	Fertile	var. communis Tsen et Lee	60	Shengfeng	China	Fertile	var. communis Tsen et Lee
27	Hongsheng	China	Fertile	var. communis Tsen et Lee	61	AijiaobaiA	China	Fertile	var. communis Tsen et Lee
28	Heiyoucai	China	Fertile	var. communis Tsen et Lee	62	Gailiangjingpin	China	Fertile	var. communis Tsen et Lee
29	Hongsheng No.6	China	Fertile	var. communis Tsen et Lee	63	Beijin510	China	Fertile	var. communis Tsen et Lee
30	Jingxuanhuangxinwu	China	Fertile	var. rosularis Tsen et Lee	64	Aizibai	China	Sterile	var. communis Tsen et Lee
31	Wuyeaijiaobai	China	Fertile	var. communis Tsen et Lee	65	Lvling	China	Fertile	var. communis Tsen et Lee
32	Aijiaobai	China	Fertile	var. communis Tsen et Lee	66	ZaoshenghuajingA	Japan	Sterile	var. communis Tsen et Lee
33	Dongyangqing No.1	Japan	Fertile	var. communis Tsen et Lee	67	QinggengP0523	Japan	Fertile	var. communis Tsen et Lee
34	Taiguozhongzi No.2	Thailand	Fertile	var. communis Tsen et Lee	68	Jinxiashi	Japan	Fertile	var. communis Tsen et Lee

“AikangqingA” is an inbred line and “Aikangqing” is the sterile line of “Aikangqin”. This is also the case for “Jinyinfeng” and “JinyinfengA”, “Aibaigeng” and “AibaigengA”, etc.

Table 2. Analysis of genetic variations generated by ISSR marker of Brassica campestris L. ssp. chinensis.

Primer	Band	PPB	He	Ne	I
820	7	66.68	0.1976	1.3591	0.2943
821	9	83.43	0.2488	1.3682	0.3791
822	8	72.84	0.2714	1.4804	0.3922
823	6	69.31	0.202	1.3832	0.3002
850	10	75.64	0.2932	1.6215	0.4135
835	8	83.32	0.2214	1.3388	0.3557
840	11	100	0.3485	1.5628	0.5516
834	7	96.2	0.3843	1.5733	0.4902
846	6	87.87	0.2957	1.4068	0.3859
848	8	86.45	0.2574	1.3742	0.3846
841	9	76.22	0.2723	1.5215	0.4474
842	10	100	0.3485	1.5628	0.5516
851	12	62	0.1495	1.2564	0.2362
809	8	71.39	0.2642	1.4667	0.3886
844	6	78.87	0.3232	1.5512	0.4742
811	8	100	0.3816	1.6493	0.5659
827	11	100	0.3752	1.6683	0.5531
810	10	100	0.3423	1.5594	0.5206
826	10	100	0.4851	1.9453	0.6781
828	9	79.98	0.3122	1.5231	0.4622
Total	173	84.21	0.2883	1.4796	0.4284

PPB: percentage of polymorphic bands; He: Nei’s (1973) genetic diversity; Ne: effective number of alleles; I: Shannon’s information index.

Figure 1. An example of ISSR analysis in Pak-choi.

Table 3. Genetic polymorphism of different geographical distribution revealed by ISSR markers of Brassica campestris L. ssp. chinensis.

Origin	Accession	Band	PPB^1	na^2	ne^3	h	I^4	Ht	Gst
China	142	173	82.66	1.8246	1.5465	0.3131	0.4614	****	****
Japan	90	167	53.89	1.5439	1.3729	0.2077	0.3037	****	****
Thailand	25	142	17.60	1.1754	1.1119	0.0658	0.0980	****	****
Total	173	173	83.82	1.8421	0.2883	1.4796	0.4284	0.2501	0.2182

PPB: Percentage of polymorphic bands; na: Observed number of alleles; ne: Effective number of alleles; h: Nei’s (1973) gene diversity; I: Shannon's Information index; Ht: Total gene diversity of all the groups; Gst: The average gene diversity among groups.

Table 4. Genetic distance value under 0.1 between different accessions of Pak-choi.

Accession	Accession	Genetic distance	Accession	Accession	Genetic distance
Xiashang	Xiadi	0.0351	Wuyueman	Wuyeaijiaobai	0.0877
AizibaiA	Aizibai	0.0877	Jinyinfeng A	Jinyinfeng	0.0702
AibaigengA	Aibaigeng	0.0702	YakangA	Yakang	0.0877
AijiaohuangA	Aujiaohuang	0.0877	Sijiqing	Zhongqiqing	0.0877
NaibaicaiA	Naibaicai	0.0877	Shanhaiqing	Sijiqing	0.0702
P0506	Jinxiashi	0.0702	Teaiqing	Sijiqing	0.0702
P0405	Xiashang	0.0702	Siyueman	Huashusijiqing	0.0526
P0405	Siyueman	0.0877

Table 5. The 10 largest genetic distance values of 10 male sterile lines with a given maintainer line (Fertility) involved in this study.

Accession	Accession	Genetic distance
AikangqingA	Beijing510	0.4561
AijiaobaiA	Shengfeng	0.4035
AijiaobaiA	Taiguozhongzi No.1	0.4035
AikangqingA	ZaoshuNo5	0.3860
NaibaicaiA	Lidun	0.3860
YakangA	Xiangqingcai	0.3860
AijiaohuangA	Jinyinfeng	0.3684
YakangA	Jinyinfeng	0.3684
AibaigengA	CK5–2	0.3680
AizibaiA	Jinyinfeng	0.3684

Figure 2. UPGMA dendrogram of 68 Pak-choi accessions based on ISSR markers.

Nei M. 1973. Analysis of diversity in subdivided populations. Proc Natl Acad Sci USA. 70:3321–3323.10.1073/pnas.70.12.3321

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