A new review and karyological analysis of Leiolepis belliana and L. boehmei (Reptilia, Agamidae) from a population in southern Thailand

Figures & data

Figure 1. General characteristics of Leiolepis belliana (A) and L. boehmei (B) from southern Thailand (scale bar indicates 5 cm).

Table 1. Mean length of the short arm chromosome (Ls), long arm chromosome (Ll), total arm chromosome (LT), centromeric index (CI), relative length (RL) and standard deviation (SD) of CI and RL from 20 metaphase cells of male and female Leiolepis belliana, 2n = 36.

Chromosome pair	Ls	Ll	LT	CI ± SD	RL ± SD	Type	Size
1*	1.117	1.342	2.459	0.546 ± 0.007	0.173 ± 0.003	m	L
2	0.929	1.266	2.195	0.577 ± 0.005	0.154 ± 0.004	m	L
3	0.766	0.957	1.723	0.555 ± 0.006	0.121 ± 0.002	m	M
4	0.761	0.868	1.629	0.533 ± 0.004	0.114 ± 0.002	m	M
5	0.682	0.750	1.432	0.524 ± 0.008	0.101 ± 0.003	m	M
6	0.495	0.547	1.042	0.525 ± 0.006	0.073 ± 0.005	m	S
7	-	-	0.432	-	0.030 ± 0.003	mi
8	-	-	0.401	-	0.028 ± 0.004	mi
9	-	-	0.377	-	0.026 ± 0.003	mi
10	-	-	0.373	-	0.026 ± 0.002	mi
11	-	-	0.357	-	0.025 ± 0.002	mi
12	-	-	0.322	-	0.022 ± 0.003	mi
13	-	-	0.305	-	0.021 ± 0.004	mi
14	-	-	0.287	-	0.020 ± 0.002	mi
15	-	-	0.252	-	0.018 ± 0.002	mi
16	-	-	0.232	-	0.016 ± 0.002	mi
17	-	-	0.213	-	0.015 ± 0.003	mi
18	-	-	0.203	-	0.014 ± 0.002	mi

*Nucleolar organizer region/NOR.

Abbreviations: m, metacentric; L, large; M, medium; S, small; mi, microchromosome.

Table 2. Mean length of the short arm chromosome (Ls), long arm chromosome (Ll), total arm chromosome (LT), centromeric index (CI), relative length (RL) and standard deviation (SD) of CI and RL from 20 metaphase cells of female Leiolepis boehmei, 2n = 36.

Chromosome pair	Ls	Ll	LT	CI ± SD	RL ± SD	Type	Size
1a*/1b	1.021	1.274	2.295	0.555 ± 0.005	0.163 ± 0.004	m	L
2	0.822	1.112	1.934	0.575 ± 0.005	0.137 ± 0.003	m	L
3	0.711	0.900	1.611	0.559 ± 0.003	0.114 ± 0.004	m	M
4	0.742	0.843	1.585	0.532 ± 0.007	0.112 ± 0.004	m	M
5	0.640	0.715	1.355	0.528 ± 0.006	0.096 ± 0.005	m	M
6	0.435	0.492	0.927	0.531 ± 0.004	0.066 ± 0.003	m	S
7	-	-	0.497	-	0.035 ± 0.003	mi
8	-	-	0.445	-	0.032 ± 0.004	mi
9	-	-	0.421	-	0.030 ± 0.002	mi
10	-	-	0.405	-	0.029 ± 0.003	mi
11	-	-	0.390	-	0.028 ± 0.004	mi
12	-	-	0.378	-	0.027 ± 0.003	mi
13	-	-	0.352	-	0.025 ± 0.003	mi
14	-	-	0.343	-	0.024 ± 0.004	mi
15	-	-	0.332	-	0.024 ± 0.003	mi
16	-	-	0.291	-	0.021 ± 0.002	mi
17	-	-	0.282	-	0.020 ± 0.002	mi
18	-	-	0.261	-	0.019 ± 0.003	mi

*Nucleolar organizer region/NOR.

Abbreviations: m, metacentric; L, large; M, medium; S, small; mi, microchromosome.

Table 3. A frequency of chromosome number per cell counts of Leiolepis belliana and L. boehmei.

	Number of cells
Number of chromosome	Male (%)	Female (%)
Leiolepis belliana
< 34	15 (11.11)	16 (10.88)
34	19 (14.07)	17 (11.57)
35	9 (6.67)	11 (7.48)
36	81 (60.00)	90 (61.23)
> 36	11 (8.15)	13 (8.84)
Total cell	135	147
L. boehmei
< 34		13 (9.85)
34		20 (15.15)
35		13 (9.85)
36		72 (54.55)
> 36		14 (10.60)
Total cell		132

Figure 2. Metaphase chromosome plates and karyotypes of male (A) and female (B) Leiolepis belliana, 2n = 36, by conventional staining technique. The secondary constrictions appear on the subtelomeric region of the long arm of the largest metacentric chromosome pair 1 (arrows). Scale bars indicate 5 µm.

Figure 3. Metaphase chromosome plates and karyotypes of male (A) and female (B) Leiolepis belliana, 2n = 36, by Ag-NOR banding technique. Nucleolar organizer regions (NORs) appear on the subtelomeric region of the long arm of the largest metacentric chromosome pair 1 (arrows). Scale bars indicate 5 µm.

Figure 4. Metaphase chromosome plates and karyotypes of female L. boehmei, 2n = 36, by conventional straining (A) and Ag-NOR banding (B). The secondary constrictions or nucleolar organizer regions (NORs) appear on the subtelomeric region of the long arm of the largest metacentric chromosome pair 1a (arrow). Scale bars indicate 5 µm.

Figure 5. Metaphase plate (A) and prophase (B) of female L. boehmei by silver-NOR banding, meiotic cell of male L. belliana during prophase by silver-NOR banding (C), and other meiotic cells of male L. belliana by conventional staining of mature sperm (D), leptotene (E), zygotene (F), pachytene (G), diakinesis (H), and metaphase II (I). Scale bars indicate 5 µm.