Meiotic behavior of a new tetraploid cytotype of Brazilian nightshade from Mount Abu, Rajasthan, India

Figures & data

Table 1. Data showing accession number, meiotic chromosome number, ploidy level and localities with altitude of different populations of Solanum seaforthianum from Mount Abu.

Accession number (PUP)	Meiotic chromosome number (n)	Ploidy level (x)	Locality with altitude (m)
59,189	12	Diploid	Guru Shikhar (1722 m)
59,188	12	Diploid	Abu Road (1220 m)
59,190	12	Diploid	Machgaon (1220 m)
59,191	24	Tetraploid	Salgaon (1220 m)
59,192	24	Tetraploid	Nakki Lake (1220 m)

Figure 1. (a) PMC at metaphase I 12 bivalents. (b) PMC at anaphase I 12:12. (c) PMC at metaphase I showing chromosomal stickiness and unoriented bivalents. (d) PMC at anaphase I showing multiple bridges. (e) PMC showing laggards at anaphase I. (f) PMC at metaphase I with the absence of equatorial plate and bivalents are scattered in the cytoplasm. (g) PMC at anaphase I showing 24:24. (h) PMC showing spindle irregularities. (i) PMC with unequal distribution of chromosomes at anaphase I. (j) PMC showing laggards at anaphase I. (k) PMC showing laggards at anaphase II. (l) PMC showing multipolarity. (m) Dyad. (n) Triads. (o) Polyad. (p) Sterile and fertile pollen grains.

Table 2. Data on meiotic abnormalities at different stages observed in four populations of Solanum seaforthianum from Mount Abu.

Accession number (PUP)	Ploidy level	Total number of PMCs observed	Chromosomal stickiness at metaphase-I (M-I)	Unoriented bivalents at metaphase-I (M-I)	Bridges at meiosis I/meiosis II	Laggards at meiosis I/meiosis II	Pollen sterility (%)
59,189	Diploid	275	42 (15.27%)	39 (14.18%)	19 (6.90%)/0	23 (8.36%)/13 (4.72%)	28%
59,190	Diploid	218	14 (6.42%)	8 (3.66%)	23 (10.55%)/0	4 (1.83%)/9 (4.12%)	15%
59,191	Tetraploid	190	4 (2.10%)	14 (7.36%)	4 (2.10%)/2(1.05%)	34 (17.89%)/18 (9.47%)	21%
59,192	Tetraploid	210	2 (0.95%)	21 (10%)	10 (4.76%)/1(0.47%)	148 (70.47%)/52 (24.76%)	33%

Table 3. Data on abnormal microsporogenesis four different populations of Solanum seaforthianum from Mount Abu.

Accession number (PUP)	Ploidy level	Total number of PMCs observed	Monads	Dyads	Triads	Tetrads	Polyads
59,189	Diploid	106	3 (2.83%)	4 (3.77%)	6 (5.66%)	91 (85.84%)	4 (3.77%)
59,190	Diploid	124	2 (1.61%)	2 (1.61%)	4 (3.22%)	114 (91.93%)	2 (1.61%)
59,191	Tetraploid	89	2 (2.24%)	3 (3.37%)	8 (8.98%)	70 (78.65%)	6 (6.74%)
59,192	Tetraploid	140	4 (2.85%)	8 (5.71%)	12 (8.57%)	109 (77.85%)	7 (5%)