Simultaneous determination of kaempferol and quercetin in Heteropogon Contortus (L.) Beauv. by validated high-performance thin layer chromatography

ABSTRACT

Presently, the fingerprint analysis of kaempferol and quercetin has been developed simultaneously via High-Performance Thin Layer Chromatography (HPTLC) from leaves, stem, and inflorescence of Heteropogon contortus. The HPTLC method for kaempferol and quercetin was optimized with the elution of toluene: ethyl acetate: formic acid (7:3:0.5 v/v) as a mobile phase. The fingerprint analysis of kaempferol and quercetin was developed at R_f values of 0.39 and 0.24, respectively, and densitometric evaluation was done at 254 nm. The linear regression data for the calibration curve of both the compounds show a good linear relationship in the concentration range of 2–12 nanogram spot⁻¹. The suggested method has been validated in terms of limit of detection (LOD) and limit of quantification (LOQ), precision, specificity, sensitivity, and accuracy. Present results show that maximum amount of kaempferol and quercetin is found in leaf extracts (35.80 and 17.01 milligram/gram of dry weight, respectively) of H. contortus.

KEYWORDS:

• Heteropogon contortus
• HPTLC
• Kaempferol
• Quercetin

Acknowledgments

The authors would like to express their profound gratitude and sincere appreciation to UGC-BSR Single Girl Child Fellowship (Award letter no. and dated. F7-152/2007 BSR; 16-12-2013) and DBT-IPLS project (Project no. BT/PR-4548/INF/22/146/2012.) sanctioned to Punjabi University, Patiala for using the facilities and financial support of this study. The authors are also thankful to Head, Department of Botany, Punjabi University, Patiala.

Conflict of Interest

There is no conflict of interest by any of the author.

Additional information

Funding

This work was supported by the UGC BSR Indira Gandhi Single Girl Child fellowship [F7-152/2007 BSR].