Changes of Blood Flow Perfusion by MR and NF-κβ Expression in the Region of Perihematoma after Experimental Intracerebral Hemorrhage: A Correlation Study

Abstract

Objective: Tostudy the correlation between dynamic changes of regional cerebral blood flow (rCBF) and nuclear factor-kappa β (NF-κβ) expression in the region of perihematoma after intracerebral hemorrhage (ICH). Methods: Twenty-seven healthy domestic cats were divided into control group (n = 3) and ICH group (n = 24) at random. They were observed at 3 hr, 6 hr, 12 hr, 24 hr, 48 hr, 72 hr, 7 days, and 15 days, respectively, for the dynamic changes of rCBF in the perihematomal region using perfusion-weighted imaging (PWI), and NF-κβ expressions using immunohistochemistry method. Results: In the perihematomal region, a noticeable decrease in rCBF had been observed within 12 hr, i.e. in a hypoperfusion state. However, cats then presented reperfusion or hyperperfusion during 12–24-hr period, and marginally hypoperfusion after 48 hr. NF-κβ positive cells were first found at 3–6 hr and peaked at 12–48 hr. At 72 hr to 7 days they showed a decreasing trend and nearly disappeared at 15 days. Conclusions: NF-κβ expression was significantly increased due to ischemia reperfusion in the perihematomal region after cerebral ischemia, and the resulting inflammatory and immune reaction were the major causes for ischemia reperfusion injury in the perihematomal region.

Keywords:

• intracerebral hemorrhage
• perfusion-weighted image
• NF-κβ
• regional cerebral blood flow