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Research Article

The taxonomic status of the headshield slug genus Nakamigawaia Kuroda and Habe, 1961 (Gastropoda: Cephalaspidea: Aglajidae), with the description of a new species from the Western Pacific

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Pages 2231-2244 | Received 23 Jun 2021, Accepted 22 Sep 2021, Published online: 11 Feb 2022

Figures & data

Table 1. List of specimens used for molecular analysis, including locality, voucher numbers and GenBank accession numbers. *Listed as Aglaja felis in GeneBank; ** listed as Nakamigawaia spiralis in GenBank. Novel sequences generated for this study are depicted in bold font

Figure 1. Live images of Nakamigawaia nakanoae sp. nov. (A, C, D) and Nakamigawaia felis (B). A, black morph, Taiwan, ZMBN 116778, animal length (L) = 10 mm (paratype). B, the Bahamas, ZMBN 91108, L = 13 mm. C–D, white dotted morph, Taiwan, ZMBN 116777, L = 8 mm (paratype).

Figure 1. Live images of Nakamigawaia nakanoae sp. nov. (A, C, D) and Nakamigawaia felis (B). A, black morph, Taiwan, ZMBN 116778, animal length (L) = 10 mm (paratype). B, the Bahamas, ZMBN 91108, L = 13 mm. C–D, white dotted morph, Taiwan, ZMBN 116777, L = 8 mm (paratype).

Figure 2. Bayesian phylogeny of Aglajidae species with a focus on the genus Nakamigawaia, based on the mitochondrial Cytochrome c oxidase subunit I (COI) gene. Numbers on branches are posterior probabilities. Tree rooted with Philine quadripartita. BM = black morph. DM = white dotted morph.

Figure 2. Bayesian phylogeny of Aglajidae species with a focus on the genus Nakamigawaia, based on the mitochondrial Cytochrome c oxidase subunit I (COI) gene. Numbers on branches are posterior probabilities. Tree rooted with Philine quadripartita. BM = black morph. DM = white dotted morph.

Figure 3. Shells of Nakamigawaia species. A, N. spiralis, holotype, NSMT-39805, ventral (upper) and dorsal (lower) views (macrophotograph), Japan. B, N. nakanoae sp. nov., micrograph of dorsal view; arrow pointing to protoconch, ZMBN 132073, animal length (L) = 7 mm (fixed length), Japan. C, N. nakanoae sp. nov., micrograph of ventral view, ZMBN 132073, L = 7 mm (fixed length), Japan. D, N. nakanoae sp. nov., micrograph of protoconch observed from ventral side of shell, ZMBN 116778, L = 10 mm, Taiwan. E, N. felis, micrograph of dorsal view, ZMBN 91108, L = 13 mm, the Bahamas. F, N. felis, micrograph of ventral view, ZMBN 91108, L = 12 mm, the Bahamas. Scale bars: a = 200 μm; b, c, e, f = 500 μm; d = 100 μm.

Figure 3. Shells of Nakamigawaia species. A, N. spiralis, holotype, NSMT-39805, ventral (upper) and dorsal (lower) views (macrophotograph), Japan. B, N. nakanoae sp. nov., micrograph of dorsal view; arrow pointing to protoconch, ZMBN 132073, animal length (L) = 7 mm (fixed length), Japan. C, N. nakanoae sp. nov., micrograph of ventral view, ZMBN 132073, L = 7 mm (fixed length), Japan. D, N. nakanoae sp. nov., micrograph of protoconch observed from ventral side of shell, ZMBN 116778, L = 10 mm, Taiwan. E, N. felis, micrograph of dorsal view, ZMBN 91108, L = 13 mm, the Bahamas. F, N. felis, micrograph of ventral view, ZMBN 91108, L = 12 mm, the Bahamas. Scale bars: a = 200 μm; b, c, e, f = 500 μm; d = 100 μm.

Figure 4. Male reproductive system of (A) Nakamigawaia nakanoae sp. nov. and (B) N. felis. A: Taiwan, ZMBN 116778, animal length (L) = 10 mm. B: the Bahamas, ZMBN 91108, L = 13 mm. ga, genital aperture; pa, penial atrium; pr, prostate. Scale bar: 1 mm.

Figure 4. Male reproductive system of (A) Nakamigawaia nakanoae sp. nov. and (B) N. felis. A: Taiwan, ZMBN 116778, animal length (L) = 10 mm. B: the Bahamas, ZMBN 91108, L = 13 mm. ga, genital aperture; pa, penial atrium; pr, prostate. Scale bar: 1 mm.