Species delimitation and genetic variation in the rare New Zealand endemic grass genus Simplicia

Figures & data

Figure 1  Map of New Zealand showing sites sampled, major cities and other locations discussed in the text. Squares, major cities; circles Simplicia laxa; triangles, Simplicia buchananii.

Table 1  Sample location, voucher and GenBank accession details for Simplicia plants used in this study.

Species	Location	Region	Lat./long.	Vouchers	GenBank ITS	GenBank ETS	GenBank trnL/F
Simplicia laxa	Williams's Bush	Wellington	39° 37′ 20.7′′ S 175° 45′ 54.02′′ E	CHR 607323	HM191437	HM191449	HM191461
Simplicia laxa	Campbell's Bush	Wellington	39° 37′ 27.8′′ S 175° 46′ 3.72′′ E	CHR 607325	HM191436	HM191448	HM191460
Simplicia laxa	Taihape Reserve	Wellington	39° 40′ 14.29′′ S 175° 48′ 18.96′′ E	CHR 607322	HM191435	HM191447	HM191459
Simplicia laxa	Ben Moi Farm	Wellington	39° 46′ 50.25′′ S 175° 51′ 59.14′′ E	CHR 607321	HM191438	HM191450	HM191462
Simplicia laxa	Honeycomb Cave	Nelson	41° 8′ 25.79′′ S 172° 11′ 42.41′′ E	AK 288071 CHR 607318	HM191442	HM191454	HM191466
Simplicia buchananii	Cobb Valley	Nelson	41° 7′ 50.96′′ S 172° 36′ 31.53′′ E	No Voucher	HM191441	HM191453	HM191463
Simplicia buchananii	West of Gordon's Pyramid	Nelson	41° 11′ 15.24′′ S 172° 40′ 3.32′′ E	AK 304801	HM191439	HM191451	HM191465
Simplicia buchananii	Cundy Creek	Nelson	41° 11′ 14.32′′ S 172° 40′ 02.28′′ E	AK 304802	HM191440	HM191452	HM191464
Simplicia laxa	Nenthorn	Otago	45° 28′ 39.6′′ S 170° 22′ 47.3′′ E	CHR 607324 CHR 607326 CHR 607327 CHR 607328 CHR 607329	HM191445	HM191457	HM191470
Simplicia laxa	Macraes	Otago	45° 25′ 35.95′′ S 170° 27′ 13.9′′ E	CHR 607319 CHR 607320	–	–	–
Simplicia laxa	Castle Rock	Otago	45° 17′ 53.92′′ S 169° 16′ 13.87′′ E	AK 304847 AK 304848	HM191443 HM191446	HM191455 HM191458	HM191467 HM191469
Simplicia laxa	Rough Ridge	Otago	45° 03′ 56.94′′ S 169° 52′ 02.80′′ E	No Voucher	HM191444	HM191456	HM191468

Table 2  Samples included in AFLP analysis.

Site	Number of samples	Number of genotypes	Number in final set
Taihape Reserve	5	1	1
Ben Moi	7	5	1
Williams's Bush	1	1	1
Campbell's Bush	1	1	1
Castle Rock	20	8	4
Macraes	18	7	6
Nenthorn	15	5	4
Rough Ridge	1	1	1
Honeycomb Cave	1	1	1
Cobb Valley	1	1	1
Cundy Stream	1	1	1
Gordon's Pyramid	1	1	1
Total	74	35	23
Note Where not all distinct genotypes observed in a population were included in the final set, those excluded differed only slightly from an included sample from the same population and grouped strongly with the included sample in preliminary analyses.

Figure 2  UPGMA dendrogram for Simplicia AFLP profiles for individual plants. Bootstrap values are shown above branches where these were ≥50%.

Figure 3  Scatterplot of principal component scores for AFLP data. ○, Otago samples; •, Honeycomb Cave sample; □, Taihape area samples; ▵, Mt Arthur area samples.

Figure 4  Most parsimonious tree for Simplicia nrDNA (ITS and ETS) sequences. Values above branches are branch lengths, those below branches are bootstrap values where these were ≥50%.

Figure 5  Most parsimonious tree for Simplicia cpDNA sequences. Scale bar is one change.