Figures & data
Table 1 List of specimens of each species collected in Buenos Aires province, Argentina.
Figure 1 Leaf surface of Verbena native species of Buenos Aires province. A, Adaxial epidermis of V. bonariensis (SEM). B, Abaxial epidermis of V. bonariensis (SEM). C, Anomocytic stomatal apparatus, adaxial epidermis of V. bonariensis (SEM). D, Adaxial epidermis of V. bonariensis (SEM). E, Adaxial epidermis of V. montevidensis (LM). F, Vascularization, abaxial epidermis of V. rigida (LM). Abbreviations: g, glandular trichomes; LM, Light Microscopy; ng, nonglandular trichomes; s, stomata; SEM, scanning electron microscopy. Bars: A, B, F, 200 µm. C, D, E, 50 µm.
Table 2 Density of stomata and glandular trichomes in the leaves of Verbena native species of Buenos Aires province, Argentina.
Figure 2 Glandular and nonglandular trichomes of Verbena native species of Buenos Aires province, leaf surface view. A, General view of abaxial epidermis of V. litoralis (ESEM). B, Detail of appressed nonglandular trichome, adaxial epidermis of V. gracilescens (ESEM). C, Long-stalked glandular trichome and nonglandular patent trichome, abaxial epidermis of V. bonariensis (SEM). D, Long-stalked glandular trichome adaxial epidermis of V. rigida (SEM). E, Long-stalked glandular trichome, adaxial epidermis of V. intermedia (ESEM). F, Four-celled head of short-stalked glandular trichome, abaxial epidermis of V. rigida (SEM). G, Multicellular head of short-stalked glandular trichome, abaxial epidermis of V. montevidensis (ESEM). Abbreviations: ESEM, environmental scanning electron microscopy; g, glandular trichome; LM, light microscopy; ng, nonglandular trichome; SEM, scanning electron microscopy. Bars: A, 250 µm. B, 150 µm. C–E, 50 µm. F, G, 10 µm.
Figure 3 Anatomical features that differentiate the leaves of Verbena native species of Buenos Aires province, Argentina. Abbreviations: Ab, Trichomes in the abaxial epidermis; Ad, Trichomes in the adaxial epidermis. The number inside the oval indicates number of cells in head of glandular trichomes.
Figure 4 Leaf transverse section of Verbena native species of Buenos Aires province (LM). A, General view of V. litoralis. B, Detail mid-vein of V. montevidensis. C, Detail of abaxial epidermis and spongy chlorenchyma of V. bonariensis. D, Detail of adaxial epidermis and palisade chlorenchyma of V. bonariensis. E, Patent nonglandular trichome in V. rigida. F, G, Nonglandular trichome with cystoliths in the basal cells of V. bonariensis. H, Short-stalked glandular trichome of V. litoralis. I, Sunken short-stalked glandular trichome of V. litoralis. J, Long-stalked glandular trichome and nonglandular trichomes of V. intermedia. K, Long-stalked glandular trichomes of V. bonariensis. L, Adaxial epidermis of V. bonariensis. Abbreviations: lg, long-stalked glandular trichome; LM, light microscopy; ps, parenchymatous sheath; s, stomata; sg, short-stalked glandular trichome; black arrow, cystoliths. Bars: A, B, F, J, L, 100 µm. C–E, K, 50 µm. G, H, I, 25 µm.
Figure 5 Stem transverse section of Verbena native species of Buenos Aires province. A, V. gracilescens. B, V. montevidensis. C, V. bonariensis. D, V. litoralis. E, V. intermedia. F, V. rigida. Bars: 500 µm.
Figure 6 Stem transverse section of Verbena native species of Buenos Aires province. A, General aspect of V. montevidensis (LM). B, Detail of stem angle in V. gracilescens (LM). C, Detail of the stem side in V. bonariensis (LM). D, Epidermis and hypodermis in V. litoralis (LM). E, Endodermis with Casparian bands in V. gracilescens (LM). F, Vascular bundle and cap of fibres in V. intermedia (LM). Abbreviations: c, collenchyma; e, endodermis; f, fibres; h, hypodermis; LM, light microscopy; s, stomata; black arrow, Casparian bands. Bars: A, 250 µm. B, C, 100 µm. D–F, 25 µm.
