Phylogenetic characterisation of marine Chroococcus-like (Cyanobacteria) strains from the Pacific region

Figures & data

Table 1. List of strains and 16S rRNA gene sequences included in the phylogenetic analyses.

Species name	Isolate	Country	GenBank	References
Chroococcus sp.	CAWBG101	New Zealand	KU513781	This study
Chroococcus sp.	CAWBG102	Cook Islands	KU513786	This study
Chroococcus sp.	CAWBG103	Tahiti	KU513785	This study
Chroococcus sp.	CAWBG110	Hawaii	KU513782	This study
Chroococcus sp.	CAWBG113	New Zealand	KU513783	This study
Chroococcus sp.	CAWBG126	New Zealand	KU513784	This study
Chroococcus sp.	JJCM Chroo-M	Czech Republic	AM710384	Jezberová (2006)
Chroococcus cf. westii	CCALA 702	Slovakia	GQ375044	Komárková et al. (2010)
Chroococcus virescens	CCALA 701	Slovakia	GQ375046	Komárková et al. (2010)
Chroococcus minutus	CCALA 055	Macedonia	GQ375047	Komárková et al. (2010)
Chroococcus sp.	CCALA 057	Slovakia	GQ375045	Komárková et al. (2010)
Limnococcus limneticus	Svet-06	Czech Republic	GQ375048	Komárková et al. (2010)
Chroococcus membraninus	CCALA 054	N/A	GQ375049	Komárková et al. (2010)
Chroococcus prescottii	JJCV Chroo-V	Czech Republic	AM710385	Jezberová (2006)
Synechocystis sp.	CCALA 701	Indonesia	GQ375043	Komárková et al. (2010)
Microcystis aeruginosa	NIES 98	Japan	D89032	Kondo et al. (1998)
Cyanothece sp.	PCC 7424	Germany	AJ000715	Nubel et al. (1997)
Acaryochloris marina	MBIC11017	USA	NR115154	N/A
Synechococcus sp.	PCC 6301	Japan	NR074309	Sugita et al. (2007)
Escherichia coli	ACN001	China	CP007442	N/A

N/A, not available.

Figure 1. Light micrographs of stationary phase cells of Chroococcus-like species isolated from A, Rangaunu Harbour, New Zealand (CAWBG101; B, Cook Islands (CAWBG102); C, Tahiti (CAWBG103); D, Hawaii (CAWBG110); E, Hokianga Harbour, New Zealand (CAWBG113); F, Motuarohia Island, New Zealand (CAWBG126). Scale bar = 20 µm.

Table 2. Mean cell sizes (n = 50) of Chroococcus spp. strains investigated in this study.

Strains	Location	Length (µm)	Width (µm)
CAWBG101	Rangaunu, New Zealand	42.5	50.0
CAWBG102	Cook Islands	39.4	49.3
CAWBG103	Tahiti	39.4	59.1
CAWBG110	Hawaii	36.4	57.6
CAWBG113	Hokianga, New Zealand	27.0	38.0
CAWBG126	Motuarohia, New Zealand	35.8	56.8

Figure 2. Phylogenetic reconstruction of Chroococcus-like strains based on 16S rRNA sequences. Clusters 1–4 are those used in Komárková et al. (2010). Numbers at nodes correspond to the Maximum Likelihood bootstrap support; values < 50% are not shown. Species names are indicated in italics followed by the GenBank Accession numbers in parentheses. Isolate numbers and original references for all sequences are detailed in Table 1. Chroococcus spp. sequences that were in Cluster 2 in Komárková et al. (2010), were not available on GenBank.

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