Analysis of HbA1c using microfluidic card (Capitainer qDBS card) as a pre-step before determination of the HbA1c value with an immunological method

Figures & data

Table 1. Testing various kinds of HbA1c elution procedures for DBS card according to visible elution rating.

	Prepared filter, µL PBS	Incubation step 1, min	Hemolysate buffer, µL	Incubation step 2, min	Incubation step 2 with shake (500 rpm), min	Vortex, second	Incubation step 3, min	Visible eluation rating 1–10 (1= red disc, 10 = white disc)
Card 1	x	x	1000	30	x	x	x	2
Card 2	x	x	1000	60	x	x	x	2
Card 3	x	x	1000	x	60	x	x	6
Card 4	x	x	1000	30	x	60	x	8
Card 5	x	x	1000	x	30	60	x	8
Card 6	x	x	1000	10	x	5	20	8
Card 7	100	10	900	x	x	5	20	10

The rating is 1 to 10, where 1 is a red disc and 10 is a white disc after all steps in the procedure.

Figure 1. Correlation between using DBS cards + cobas (Y-axis) and assigned values using EDTA blood sample + Capillarys 3 (X-axis).

Figure 2. Analytical performance specifications with acceptance criteria for HbA1c using DBS card compared with assigned values from Capillarys 3 in mmol/mol.

Figure 3. Method comparison for HbA1c analyzed at Capillarys 3 Tera, cobas 6000 (c501) and Capitainer 2^nd gen DBS cards analyzed at cobas 6000 (c501) in mmol/mol.

Figure 4. Method comparison with acceptance criteria for HbA1c using cobas 6000 with and without DBS card, compared with assigned values from Capillarys 3 in mmol/mol.